Ontology highlight
ABSTRACT:
SUBMITTER: Pham QN
PROVIDER: S-EPMC7161830 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Pham Quang Nghia QN Trinh Kieu The Loan KTL Jung Seung Won SW Lee Nae Yoon NY
Biotechnology and bioengineering 20180606 9
We demonstrate the integration of DNA amplification and detection functionalities developed on a lab-on-a-chip microdevice utilizing solid-phase polymerase chain reaction (SP-PCR) for point-of-need (PON) DNA analyses. First, the polycarbonate microdevice was fabricated by thermal bonding to contain microchambers as reservoirs for performing SP-PCR. Next, the microchambers were subsequently modified with polyethyleneimine and glutaraldehyde for immobilizing amine-modified forward primers. During ...[more]