Identification of GBF1 as a cellular factor required for hepatitis E virus RNA replication.
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ABSTRACT:
SUBMITTER: Farhat R
PROVIDER: S-EPMC7162332 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
Identification of GBF1 as a cellular factor required for hepatitis E virus RNA replication.
Farhat Rayan R Ankavay Maliki M Lebsir Nadjet N Gouttenoire Jérôme J Jackson Catherine L CL Wychowski Czeslaw C Moradpour Darius D Dubuisson Jean J Rouillé Yves Y Cocquerel Laurence L
Cellular microbiology 20171117 1
The hepatitis E virus (HEV) genome is a single-stranded, positive-sense RNA that encodes three proteins including the ORF1 replicase. Mechanisms of HEV replication in host cells are unclear, and only a few cellular factors involved in this step have been identified so far. Here, we used brefeldin A (BFA) that blocks the activity of the cellular Arf guanine nucleotide exchange factors GBF1, BIG1, and BIG2, which play a major role in reshuffling of cellular membranes. We showed that BFA inhibits H ...[more]
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