Dataset Information

Divergent effect of fast- and slow-releasing H₂S donors on boar spermatozoa under oxidative stress.

ABSTRACT: Hydrogen sulphide (H₂S) is involved in the physiology and pathophysiology of different cell types, but little is known about its role in sperm cells. Because of its reducing properties, we hypothesise that H₂S protects spermatozoa against the deleterious effects of oxidative stress, a condition that is common to several male fertility disorders. This study aimed i) to determine the total antioxidant capacities of Na₂S and GYY4137, which are fast- and slow-releasing H₂S donors, respectively, and ii) to test whether H₂S donors are able to protect spermatozoa against oxidative stress. We found that Na₂S and GYY4137 show different antioxidant properties, with the total antioxidant capacity of Na₂S being mostly unstable and even undetectable at 150?µM. Moreover, both H₂S donors preserve sperm motility and reduce acrosome loss, although the effects were both dose and donor dependent. Within the range of concentrations tested (3-300?µM), GYY4137 showed positive effects on sperm motility, whereas Na₂S was beneficial at the lowest concentration but detrimental at the highest. Our findings show that Na₂S and GYY4137 have different antioxidant properties and suggest that both H₂S donors might be used as in vitro therapeutic agents against oxidative stress in sperm cells, although the optimal therapeutic range differs between the compounds.

SUBMITTER: Pintus E

PROVIDER: S-EPMC7162918 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Divergent effect of fast- and slow-releasing H2S donors on boar spermatozoa under oxidative stress.

Pintus Eliana E Jovičić Marija M Kadlec Martin M Ros-Santaella José Luis JL

Scientific reports 20200416 1

Hydrogen sulphide (H2S) is involved in the physiology and pathophysiology of different cell types, but little is known about its role in sperm cells. Because of its reducing properties, we hypothesise that H2S protects spermatozoa against the deleterious effects of oxidative stress, a condition that is common to several male fertility disorders. This study aimed i) to determine the total antioxidant capacities of Na2S and GYY4137, which are fast- and slow-releasi ...[more]

PMID: 32300246

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Project description:AimsTo determine how the accumulation of drug in mice bearing an extra-hepatic tumor and its therapeutic efficacy are affected by the type of PEGylated liposomal doxorubicin used, treatment modality, and rate of drug release from the liposomes, when combined with radiofrequency (RF) ablation.Materials and methodsTwo nano-drugs, both long-circulating PEGylated doxorubicin liposomes, were formulated: (1) PEGylated doxorubicin in thermosensitive liposomes (PLDTS), having a burst-type fast drug release above the liposomes' solid ordered to liquid disordered phase transition (at 42°C), and (2) non-thermosensitive PEGylated doxorubicin liposomes (PLDs), having a slow and continuous drug release. Both were administered intravenously at 8 mg/kg doxorubicin dose to tumor-bearing mice. Animals were divided into 6 groups: no treatment, PLD, RF, RF+PLD, PLDTS, and PLDTS+RF, for intra-tumor doxorubicin deposition at 1, 24, and 72 h post-injection (in total 41, mice), and 31 mice were used for randomized survival studies.ResultsNon-thermosensitive PLD combined with RF had the least tumor growth and the best end-point survival, better than PLDTS+RF (p<0.005) or all individual therapies (p<0.001). Although at 1 h post-treatment the greatest amount of intra-tumoral doxorubicin was seen following PLDTS+RF (p<0.05), by 24 and 72 h the greatest doxorubicin amount was seen for PLD+RF (p<0.05); in this group the tumor also has the longest exposure to doxorubicin.ConclusionOptimizing therapeutic efficacy of PLD requires a better understanding of the relationship between the effect of RF on tumor microenvironment and liposome drug release profile. If drug release is too fast, the benefit of changing the microenvironment by RF on tumor drug localization and therapeutic efficacy may be much smaller than for PLDs having slow and temperature-independent drug release. Thus the much longer circulation time of doxorubicin from PLD than from PLDTS may be beneficial in many therapeutic instances, especially in extra-hepatic tumors.

Dataset Information

Divergent effect of fast- and slow-releasing H₂S donors on boar spermatozoa under oxidative stress.

Publications

Divergent effect of fast- and slow-releasing H<sub>2</sub>S donors on boar spermatozoa under oxidative stress.

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