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A near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by 18F-positron emitting tomography and fluorescence.

ABSTRACT: PURPOSE:Knowing the precise flow of cerebrospinal fluid (CSF) is important in the management of multiple neurological diseases. Technology for non-invasively quantifying CSF flow would allow for precise localization of injury and assist in evaluating the viability of certain devices placed in the central nervous system (CNS). METHODS:We describe a near-infrared fluorescent dye for accurately monitoring CSF flow by positron emission tomography (PET) and fluorescence. IR-783, a commercially available near-infrared dye, was chemically modified and radiolabeled with fluorine-18 to give [18F]-IR783-AMBF3. [18F]-IR783-AMBF3 was intrathecally injected into the rat models with normal and aberrant CSF flow and evaluated by the fluorescence and PET/MRI or PET/CT imaging modes. RESULTS:IR783-AMBF3 was clearly distributed in CSF-containing volumes by PET and fluorescence. We compared IR783-AMBF3 (fluorescent at 778/793?nm, ex/em) to a shorter-wavelength, fluorescein equivalent (fluorescent at 495/511?nm, ex/em). IR783-AMBF3 was superior for its ability to image through blood (hemorrhage) and for imaging CSF-flow, through-skin, in subdural-run lumboperitoneal shunts. IR783-AMBF3 was safe under the tested dosage both in vitro and in vivo. CONCLUSION:The superior imaging properties of IR783-AMBF3 could lead to enhanced accuracy in the treatment of patients and would assist surgeons in non-invasively diagnosing diseases of the CNS.

SUBMITTER: Guo H

PROVIDER: S-EPMC7163004 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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A near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by <sup>18</sup>F-positron emitting tomography and fluorescence.

Guo Hua H Kommidi Harikrishna H Lekaye Carl C CC Koutcher Jason J Judenhofer Martin S MS Cherry Simon R SR Wu Amy P AP Akin Oguz O Souweidane Mark M MM Aras Omer O Zhu Zhaohui Z Ting Richard R

EJNMMI research 20200416 1

<h4>Purpose</h4>Knowing the precise flow of cerebrospinal fluid (CSF) is important in the management of multiple neurological diseases. Technology for non-invasively quantifying CSF flow would allow for precise localization of injury and assist in evaluating the viability of certain devices placed in the central nervous system (CNS).<h4>Methods</h4>We describe a near-infrared fluorescent dye for accurately monitoring CSF flow by positron emission tomography (PET) and fluorescence. IR-783, a comm ...[more]

PMID: 32301036

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Dataset Information

A near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by 18F-positron emitting tomography and fluorescence.

Publications

A near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by <sup>18</sup>F-positron emitting tomography and fluorescence.

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