Ontology highlight
ABSTRACT:
SUBMITTER: Chakraborti S
PROVIDER: S-EPMC7166090 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Chakraborti Soumyananda S Balakrishnan Dhanasekaran D Trotter Alexander J AJ Gittens William H WH Yang Ally W H AWH Jolma Arttu A Paterson Joy R JR Świątek Sylwia S Plewka Jacek J Curtis Fiona A FA Bowers Laura Y LY Pålsson Lars-Olof LO Hughes Timothy R TR Taube Michał M Kozak Maciej M Heddle Jonathan G JG Sharples Gary J GJ
The Biochemical journal 20200401 7
We report the identification and characterization of a bacteriophage λ-encoded protein, NinH. Sequence homology suggests similarity between NinH and Fis, a bacterial nucleoid-associated protein (NAP) involved in numerous DNA topology manipulations, including chromosome condensation, transcriptional regulation and phage site-specific recombination. We find that NinH functions as a homodimer and is able to bind and bend double-stranded DNA in vitro. Furthermore, NinH shows a preference for a 15 bp ...[more]