Antiviral activity of porcine interferon omega 7 against foot-and-mouth disease virus in vitro.

ABSTRACT: Foot-and-mouth disease (FMD) is a disease of worldwide economic importance, and vaccines play an important role in preventing FMDV outbreaks. However, new control strategies are still needed since FMDV outbreaks still occur in some disease-free countries. Currently, interferon (IFN)-based strategies have been demonstrated to be an efficient biotherapeutic option against FMDV; however, interferon omega (IFN-?) has not yet been assessed in this capacity. Thus, this study evaluated the antiviral activity of porcine IFN omega 7 (PoIFN-?7) against FMDV. After the PoIFN-?7 was expressed and purified, cell proliferation assays and quantitative real-time reverse transciption-polymerase chain reaction were used to evaluate the effective anti-cytopathic concentration of PoIFN-?7 and its effectiveness pre- and post-infection with FMDV in swine kidney cells (IBRS-2). Results showed the rHis-PoIFN-?7 fusion protein was considerably expressed using Escherichia coli BL21 (DE3) strain, and the recombinant protein exhibited significant in vitro protection against FMDV, including two strains belonging to type O and A FMDV, respectively. In addition, PoIFN-?7 upregulated the transcription of Mx1, ISG15, OAS1, and PKR genes. These findings indicated that IFN-? has the potential for serving as a useful therapeutic agent to prevent FMDV or other viral outbreaks in pigs.

SUBMITTER: Li SF 

PROVIDER: S-EPMC7166596 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature