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The Influence of Serum Sample Storage Conditions on Selected Laboratory Parameters Related to Oxidative Stress: A Preliminary Study.

ABSTRACT: The present work aims at accessing the stability of biological material stored for diagnostic and scientific purposes. The influence of the temperature, storage time, and cyclic thawing on concentration stability of selected oxidative stress parameters in human serum was investigated. The study group consisted of 20 serum samples collected from healthy volunteers aged 18-52. The parameters whose reference ranges were not determined and to which validated determination methods did not correspond were examined by manual methods (FRAP and AOPP). Automatic methods were used to determine routine laboratory tests (albumin, total protein, bilirubin, uric acid) using the Konelab 20i® analyzer. The samples were stored at various temperatures (room temperature, 4 °C, -20 °C, -80 °C) for max 6 months and were subjected to cyclic thawing at 1 month intervals. In order to check whether any differences between the concentrations of the studied parameters existed when the samples were stored in various conditions, the paired Student t-test or Wilcoxon test and comparison to desirable bias were applied. Based on the obtained results, it was found that the temperature and time of serum sample storage significantly affected the stability of the analyzed parameters and determined different shelf lives of serum samples for oxidative stress examination. Therefore, continuing the investigation concerning the impact of storage conditions on various serum parameters seems justified due to the discrepancy between the individual results obtained by different researchers and the inconsistencies between the results of scientific research and the applicable recommendations.

SUBMITTER: Pawlik-Sobecka L

PROVIDER: S-EPMC7168228 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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The Influence of Serum Sample Storage Conditions on Selected Laboratory Parameters Related to Oxidative Stress: A Preliminary Study.

Pawlik-Sobecka Lilla L Sołkiewicz Katarzyna K Kokot Izabela I Kiraga Aleksandra A Płaczkowska Sylwia S Schlichtinger Agnieszka Matylda AM Kratz Ewa Maria EM

Diagnostics (Basel, Switzerland) 20200119 1

The present work aims at accessing the stability of biological material stored for diagnostic and scientific purposes. The influence of the temperature, storage time, and cyclic thawing on concentration stability of selected oxidative stress parameters in human serum was investigated. The study group consisted of 20 serum samples collected from healthy volunteers aged 18-52. The parameters whose reference ranges were not determined and to which validated determination methods did not correspond ...[more]

PMID: 31963920

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Project description:Simple Summary The consumption of fermented milk products can have health benefits, particularly for those that suffer from lactose intolerance. Condition that is usually caused by insufficient production in the intestinal epithelium of lactase, the enzyme that facilitates the digestion of milk sugar (lactose). The lactose originally present in fermented beverages undergoes fermentation to lactic acid, so its concentration is lower than in processed milk. The aim of this study was to determine the effects of the storage time of fermented beverages made from sheep’s milk on the lactose content of kefir and yogurts inoculated with various starter cultures. The drinks were made from sheep’s milk with natural fat content. A sensory evaluation of the products was carried out to determine the most favorable date of consumption in terms of lactose concentration and organoleptic values. Effects of both inoculation type and beverage storage time were shown for all the parameters. Analysis showed that the optimal storage time for natural yogurt was 21 days. If kefir is to have its optimal taste, storage for more than fourteen days is not advised. Abstract The aim of the research was to evaluate lactose content and rheological, physical, chemical, and organoleptic parameters during the storage of fermented beverages made from sheep’s milk. The research was carried out on natural, probiotic, and Greek-type yogurts, as well as kefir. The products were made using the thermostat method from the milk of 42 East Frisian sheep in the middle lactation period, in duplicate. Lactose contents, active and titratable acidity, color by the L*a*b*C*h* system, and rheological parameters (hardness, consistency, consistency, and viscosity) were tested, and organoleptic assessments were carried out on the first, seventh, fourteenth, and twenty-first days of storing the drinks at 4 °C. Of all drinks, the highest reduction in lactose after 21 days of storage was found to occur in kefir (52% reduction) and, among the yogurts, in the Greek yogurt (41% reduction). The product with the lowest lactose content, regardless of the storage period, was kefir. This indicates that kefir is more suitable than yogurt for people with partial lactose intolerance. Effects of both inoculation type and beverage storage time were shown to exist for all parameters. It was also found that kefirs suffered deterioration in most rheological parameters and, in general organoleptic evaluation in the final period of storage. Based on our analysis, the optimal storage time for natural yogurts and sheep’s milk kefirs at 4 °C was 21 and 14 days, respectively.

Dataset Information

The Influence of Serum Sample Storage Conditions on Selected Laboratory Parameters Related to Oxidative Stress: A Preliminary Study.

Publications

The Influence of Serum Sample Storage Conditions on Selected Laboratory Parameters Related to Oxidative Stress: A Preliminary Study.

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