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Transient Transfection of the Zoonotic Parasite Babesia microti.

ABSTRACT: The development of genetic manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not been established for Babesia microti. Here, we report the first successful transient transfection of B. microti. The plasmids containing the firefly luciferase reporter gene were transfected into B. microti by an AMAXA 4D Nucleofection system. Twenty-four-hour synchronization, the 5'-actin promoter, program FA100, and 50 ?g of plasmid DNA constituted the best conditions for the transient transfection of B. microti. This finding is the first step towards a stable transfection method for B. microti, which may contribute to a better understanding of the biology of the parasite.

SUBMITTER: Liu M

PROVIDER: S-EPMC7169379 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Transient Transfection of the Zoonotic Parasite Babesia microti.

Liu Mingming M Ji Shengwei S Rizk Mohamed Abdo MA Adjou Moumouni Paul Franck PF Galon Eloiza May EM Li Jixu J Li Yongchang Y Zheng Weiqing W Benedicto Byamukama B Tumwebaze Maria Agnes MA Asada Masahito M Xuan Xuenan X

Pathogens (Basel, Switzerland) 20200210 2

The development of genetic manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not been established for Babesia microti. Here, we report the first successful transient transfection of B. microti. The plasmids containing the firefly luciferase reporter gene were transfected into B. microti by an AMAXA 4D Nucleofection system. Twenty-four-hour synchronization, the 5'-actin promoter, program FA100, and 50 μg of ...[more]

PMID: 32050586

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