MiR?539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1.

ABSTRACT: MicroRNA (miR)?539 has inhibitory effects on certain types of cancer, but its role in pancreatic cancer (PCa) remains unclear. The present study investigated the effects of miR?539 on PCa, and aimed to determine possible therapeutic targets for the treatment of PCa. The expression of miR?539 in PCa tissues, paired normal adjacent tissues and PCa cell lines (CAPAN?2, BxPC3, CFPAC1, SW1990 and PANC1), and human non?cancerous pancreatic cells (hTRET?HPNE) was determined and compared. The effects of upregulation and downregulation of miR?539 on proliferation, apoptosis, cell cycle, invasion, migration and epithelial?mesenchymal transition (EMT) of PCa cells were investigated. Additionally, the target gene of miR?539 was predicted and its effects on PCa cells were further investigated. The results revealed low expression of miR?539 in PCa tissues and cell lines. Additionally, increasing miR?539 expression inhibited the proliferation, migration, invasion and EMT of PCa cells and induced apoptosis by blocking G1 phase of the cell cycle, while reducing miR?539 expression had the opposite results. Furthermore, specificity protein 1 (SP1) was found to be the target gene of miR?539. SP1 promoted the proliferation, migration, invasion and EMT transformation of PCa cells, but these effects were reversed by high expression of miR?539. Additionally, miR?539 suppressed the proliferation, metastasis, invasion and EMT transformation of PCa cells through targeting SP1. Therefore, miR?539 overexpression may contribute toward development of novel therapeutic strategies for PCa in the future.

SUBMITTER: Xue L 

PROVIDER: S-EPMC7169848 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature