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Heavy reliance on plants for Romanian cave bears evidenced by amino acid nitrogen isotope analysis.

ABSTRACT: Heavy reliance on plants is rare in Carnivora and mostly limited to relatively small species in subtropical settings. The feeding behaviors of extinct cave bears living during Pleistocene cold periods at middle latitudes have been intensely studied using various approaches including isotopic analyses of fossil collagen. In contrast to cave bears from all other regions in Europe, some individuals from Romania show exceptionally high ?15N values that might be indicative of meat consumption. Herbivory on plants with high ?15N values cannot be ruled out based on this method, however. Here we apply an approach using the ?15N values of individual amino acids from collagen that offsets the baseline ?15N variation among environments. The analysis yielded strong signals of reliance on plants for Romanian cave bears based on the ?15N values of glutamate and phenylalanine. These results could suggest that the high variability in bulk collagen ?15N values observed among cave bears in Romania reflects niche partitioning but in a general trophic context of herbivory.

SUBMITTER: Naito YI

PROVIDER: S-EPMC7170912 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Heavy reliance on plants for Romanian cave bears evidenced by amino acid nitrogen isotope analysis.

Naito Yuichi I YI Meleg Ioana N IN Robu Marius M Vlaicu Marius M Drucker Dorothée G DG Wißing Christoph C Hofreiter Michael M Barlow Axel A Bocherens Hervé H

Scientific reports 20200420 1

Heavy reliance on plants is rare in Carnivora and mostly limited to relatively small species in subtropical settings. The feeding behaviors of extinct cave bears living during Pleistocene cold periods at middle latitudes have been intensely studied using various approaches including isotopic analyses of fossil collagen. In contrast to cave bears from all other regions in Europe, some individuals from Romania show exceptionally high δ<sup>15</sup>N values that might be indicative of meat consumpt ...[more]

PMID: 32313007

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Project description:Human activities have substantially increased atmospheric nitrogen (N) deposition in ecosystems worldwide, often leading to higher plant quality for herbivores and greater herbivory. Predators frequently suppress herbivores and indirectly benefit plants via "trophic cascades", and the strength of these interactions can also depend on N availability. However, the evidence for N deposition effects on cascades primarily comes from studies of high-level N deposition. Most terrestrial ecosystems currently receive elevated, but low-level N deposition, and it is unclear whether this subtle N enrichment has any effect on cascades. Here, I asked whether low-level N deposition alters a trophic cascade from black bears to plants in Colorado. In this ecological network, bears indirectly benefit plants by consuming ants and suppressing positive effects of ants on herbivores. Using a three year N enrichment experiment, I assessed changes in this cascade by measuring plant and arthropod responses to simulated N deposition, bear damage to ant nests, and the presence of mutualist herbivores and ants. I found that low-level N enrichment and bears had interacting effects on plant reproduction. In ambient N conditions, bears indirectly increased plant reproduction by causing ant nests to become inactive and suppressing positive ant effects on herbivores that were detrimental for plants. Yet, bear-induced ant nest inactivity had no effect on plant reproduction in N-enriched conditions. When N was added, ants had greater positive effects on herbivores, but herbivores had weak effects on plants, potentially because plants were more resistant to herbivores. Ultimately, the results indicate that N enrichment strengthened resource control of the community and weakened plant-herbivore interactions and the cascade from bears to plants. This study suggests that common rates of low-level N deposition are changing the strength of trophic cascades and may have already altered resource versus consumer control of ecological community structure in many ecosystems.

Project description:BackgroundThe increasing human impact in Romanian caves raises the urgency of publishing a correct database of the strictly-adapted cave fauna. Previous attempts at indexing cave fauna and classifying caves by using their fauna opened many questions regarding the use of an incomplete list of cave species and mixed lists of troglobionts/stygobionts with troglophiles/stygophiles for ranking caves with priority for protection. It has also become obvious that there is a need to publish a list of Romanian cave species that are under threat. Cave species in Romania (and elsewhere) are endemic on small ranges, are unique and must be considered as important units for conservation. A cave must be equally protected if it has one or more rare and strictly endemic cave species. Although not exhaustive, we here provide the first checklist of Romanian troglobionts/stygobionts developed in the framework of the DARKFOOD and GROUNDWATERISK projects, coordinated by the "Emil Racovita" Institute of Speleology, Cluj-Napoca, Romania. The GIS application was used to complement the checklist of cave species with data on caves and surface environments above the caves. Until complete data on species diversity and population sizes are made available for each cave, measures of conservation can be implemented, based on the presence/absence of cave species, while classifications of caves for protection, based on the number of species, must be avoided. We also propose a list of Romanian caves with fauna that are under threat and a tentative Red List of Romanian troglobiont/stygobionts.New informationThis is the first database with identified troglobiont and stygobiont species of Romania, with a critical analysis of their distribution inside the country. A list of caves that need protection for their rare and unique species and a tentative Red List of Romanian cave fauna are also added. A total of 173 species were identified, of which 77 troglobionts and 96 stygobionts are currently registered in 366 caves. The database is divided into two parts, one part with a list of troglobionts, their revised systematic position, cave name, cave code and geographic region; and the second part with the same information on stygobionts. The database represents the contribution of many active researchers, who are the authors of this paper and of review publications of many other authors of the "Emil Racoviță" Institute of Speleology.

Project description:Background and aimsMycoheterotrophy entails plants meeting all or a portion of their carbon (C) demands via symbiotic interactions with root-inhabiting mycorrhizal fungi. Ecophysiological traits of mycoheterotrophs, such as their C stable isotope abundances, strongly correlate with the degree of species' dependency on fungal C gains relative to C gains via photosynthesis. Less explored is the relationship between plant evolutionary history and mycoheterotrophic plant ecophysiology. We hypothesized that the C and nitrogen (N) stable isotope compositions, and N concentrations of fully and partially mycoheterotrophic species differentiate them from autotrophs, and that plant family identity would be an additional and significant explanatory factor for differences in these traits among species. We focused on mycoheterotrophic species that associate with ectomycorrhizal fungi from plant families Ericaceae and Orchidaceae.MethodsPublished and unpublished data were compiled on the N concentrations, C and N stable isotope abundances (δ(13)C and δ(15)N) of fully (n = 18) and partially (n = 22) mycoheterotrophic species from each plant family as well as corresponding autotrophic reference species (n = 156). These data were used to calculate site-independent C and N stable isotope enrichment factors (ε). Then we tested for differences in N concentration, (13)C and (15)N enrichment among plant families and trophic strategies.Key resultsWe found that in addition to differentiating partially and fully mycoheterotrophic species from each other and from autotrophs, C and N stable isotope enrichment also differentiates plant species based on familial identity. Differences in N concentrations clustered at the plant family level rather than the degree of dependency on mycoheterotrophy.ConclusionsWe posit that differences in stable isotope composition and N concentrations are related to plant family-specific physiological interactions with fungi and their environments.

Dataset Information

Heavy reliance on plants for Romanian cave bears evidenced by amino acid nitrogen isotope analysis.

Publications

Heavy reliance on plants for Romanian cave bears evidenced by amino acid nitrogen isotope analysis.

Similar Datasets

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