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NusA directly interacts with antitermination factor Q from phage ?.


ABSTRACT: Antitermination (AT) is a ubiquitous principle in the regulation of bacterial transcription to suppress termination signals. In phage ? antiterminator protein Q controls the expression of the phage's late genes with loading of ?Q onto the transcription elongation complex halted at a ?-dependent pause requiring a specific DNA element. The molecular basis of ?Q-dependent AT and its dependence on N-utilization substance (Nus) A is so far only poorly understood. Here we used solution-state nuclear magnetic resonance spectroscopy to show that the solution structure of ?Q is in agreement with the crystal structure of an N-terminally truncated variant and that the 60 residues at the N-terminus are unstructured. We also provide evidence that multidomain protein NusA interacts directly with ?Q via its N-terminal domain (NTD) and the acidic repeat (AR) 2 domain, with the ?Q:NusA-AR2 interaction being able to release NusA autoinhibition. The binding sites for NusA-NTD and NusA-AR2 on ?Q overlap and the interactions are mutually exclusive with similar affinities, suggesting distinct roles during ?Q-dependent AT, e.g. the ?Q:NusA-NTD interaction might position NusA-NTD in a way to suppress termination, making NusA-NTD repositioning a general scheme in AT mechanisms.

SUBMITTER: Dudenhoeffer BR 

PROVIDER: S-EPMC7171158 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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NusA directly interacts with antitermination factor Q from phage λ.

Dudenhoeffer Benjamin R BR   Borggraefe Jan J   Schweimer Kristian K   Knauer Stefan H SH  

Scientific reports 20200420 1


Antitermination (AT) is a ubiquitous principle in the regulation of bacterial transcription to suppress termination signals. In phage λ antiterminator protein Q controls the expression of the phage's late genes with loading of λQ onto the transcription elongation complex halted at a σ-dependent pause requiring a specific DNA element. The molecular basis of λQ-dependent AT and its dependence on N-utilization substance (Nus) A is so far only poorly understood. Here we used solution-state nuclear m  ...[more]

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