CNV analysis of Meishan pig by next-generation sequencing and effects of AHR gene CNV on pig reproductive traits.

ABSTRACT: Background:Reproductive performance of livestock is an economically important aspect of global food production. The Chinese Meishan pig is a prolific breed, with an average of three to five more piglets per litter than European breeds; however, the genetic basis for this difference is not well understood. Results:In this study, we investigated copy number variations (CNVs) of 32 Meishan pigs and 29 Duroc pigs by next-generation sequencing. A genome-wide analysis of 61 pigs revealed 12,668 copy number variable regions (CNVRs) that were further divided into three categories based on copy number (CN) of the whole population, i.e., gain (n?=?7,638), and loss (n?=?5,030) CNVRs. We then compared Meishan and Duroc pigs and identified 17.17?Mb of 6,387 CNVRs that only existing in Meishan pigs CNVRs that overlapped the reproduction-related gene encoding the aryl hydrocarbon receptor (AHR) gene. We found that normal AHR CN was more frequent than CN loss in four different pig breeds. An association analysis showed that AHR CN had a positive effect on litter size (P?

SUBMITTER: Zheng X 

PROVIDER: S-EPMC7171861 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature