Unknown

Dataset Information

0

Iron competition triggers antibiotic biosynthesis in Streptomyces coelicolor during coculture with Myxococcus xanthus.


ABSTRACT: Microbial coculture to mimic the ecological habitat has been suggested as an approach to elucidate the effect of microbial interaction on secondary metabolite biosynthesis of Streptomyces. However, because of chemical complexity during coculture, underlying mechanisms are largely unknown. Here, we found that iron competition triggered antibiotic biosynthesis in Streptomyces coelicolor during coculture with Myxococcus xanthus. During coculture, M. xanthus enhanced the production of a siderophore, myxochelin, leading M. xanthus to dominate iron scavenging and S. coelicolor to experience iron-restricted conditions. This chemical competition, but not physical contact, activated the actinorhodin biosynthetic gene cluster and the branched-chain amino acid degradation pathway which imply the potential to produce precursors, along with activation of a novel actinorhodin export system. Furthermore, we found that iron restriction increased the expression of 21 secondary metabolite biosynthetic gene clusters (smBGCs) in other Streptomyces species. These findings suggested that the availability for key ions stimulates specific smBGCs, which had the potential to enhance secondary metabolite biosynthesis in Streptomyces.

SUBMITTER: Lee N 

PROVIDER: S-EPMC7174319 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Iron competition triggers antibiotic biosynthesis in Streptomyces coelicolor during coculture with Myxococcus xanthus.

Lee Namil N   Kim Woori W   Chung Jinkyoo J   Lee Yongjae Y   Cho Suhyung S   Jang Kyoung-Soon KS   Kim Sun Chang SC   Palsson Bernhard B   Cho Byung-Kwan BK  

The ISME journal 20200128 5


Microbial coculture to mimic the ecological habitat has been suggested as an approach to elucidate the effect of microbial interaction on secondary metabolite biosynthesis of Streptomyces. However, because of chemical complexity during coculture, underlying mechanisms are largely unknown. Here, we found that iron competition triggered antibiotic biosynthesis in Streptomyces coelicolor during coculture with Myxococcus xanthus. During coculture, M. xanthus enhanced the production of a siderophore,  ...[more]

Similar Datasets

| S-EPMC3818858 | biostudies-literature
| S-EPMC3924187 | biostudies-literature
| S-EPMC2276382 | biostudies-literature
| S-EPMC95220 | biostudies-literature
| S-EPMC2444045 | biostudies-literature
| S-EPMC7484181 | biostudies-literature
| S-EPMC5335527 | biostudies-literature
| S-EPMC94083 | biostudies-literature
| S-EPMC3986340 | biostudies-literature
| S-EPMC3134472 | biostudies-literature