Dataset Information

Cadmium and Mercury phytostabilization from soil using Miscanthus × giganteus.

ABSTRACT: The determination of the effects of cadmium and mercury on the growth, biomass productivity and phytoremediation potential of Miscanthus × giganteus (MxG) grown on contaminated soil was the main aim of this paper. The use of bioenergy plants as an innovative strategy in phytotechnology gives additional benefits, including mitigation and adaptation to climate change, and soil remediation without affecting soil fertility. An experiment was set up as a randomized complete block design with the treatments varied in concentrations of Cd (0, 10 and 100?mg?kg-1 soil) and Hg (0, 2 and 20?mg?kg-1 soil) added to the soil. Three vegetative years were studied. Yield values ranged from 6.3-15.5 tDM ha-1, cadmium concentration in plants varied from 45-6758?µg?kg-1 and Hg varied from 8.7-108.9?µg?kg-1. Values between treatments and years were significantly different. MxG can accumulate and remove very modest amount (up to 293.8?µg?Cd and 4.7?µg Hg) per pot per year in aboveground biomass. Based on this data it can be concluded that MxG, as a valuable energy crop, is a potential candidate for the phytostabilization and biomass production on soils contaminated with Cd and Hg moderately.

SUBMITTER: Zgorelec Z

PROVIDER: S-EPMC7174394 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Cadmium and Mercury phytostabilization from soil using Miscanthus × giganteus.

Zgorelec Zeljka Z Bilandzija Nikola N Knez Kristina K Galic Marija M Zuzul Silva S

Scientific reports 20200421 1

The determination of the effects of cadmium and mercury on the growth, biomass productivity and phytoremediation potential of Miscanthus × giganteus (MxG) grown on contaminated soil was the main aim of this paper. The use of bioenergy plants as an innovative strategy in phytotechnology gives additional benefits, including mitigation and adaptation to climate change, and soil remediation without affecting soil fertility. An experiment was set up as a randomized complete block design with the trea ...[more]

PMID: 32317673

Similar Datasets

Project description:Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from different Miscanthus x giganteus tissues (including leaves, flowers, and rhizomes). The high depth of the resulting datasets enabled us to examine in detail critical small RNA features such as size distribution, tissue-specific regulation and sequence conservation between different organs in this species. We also developed database resources and a dedicated website (http://smallrna.udel.edu/) with computational tools for allowing other users to identify new miRNAs or siRNAs involved in specific regulatory pathways, verify the degree of conservation of these sequences in other plant species and map small RNAs on genes or larger regions of the genome under study. Small RNA libraries were derived from leaves, flowers and rhizomes of Miscanthus x giganteus. Each tissue represented a mixture of developmental stages, with a bias towards those from early, actively differentiating cells. For rhizomes, the apical tip portion was collected. Total RNA was isolated using the Plant RNA Purification Reagent (Invitrogen) and submitted to Illumina (Hayward, CA, http://www.illumina.com) for small RNA library construction using approaches described in (Lu et al., 2007) with minor modifications. The small RNA libraries were sequenced with the Sequencing-By-Synthesis (SBS) technology by Illumina. PERL scripts were designed to remove the adapter sequences and determine the abundance of each distinct small RNA. We thank Steven Moose for providing the plant material and Kan Nobuta for assistance with the computational methods.

Project description:Miscanthus × giganteus is a promising high-yielding perennial plant to meet growing bioenergy demands; however, the degree to which the soil microbiome affects its nitrogen cycling and subsequently, biomass yield remains unclear. In this study, we hypothesize that contributions of metabolically active soil microbial membership may be underestimated with DNA-based approaches. We assessed the response of the soil microbiome to nitrogen availability in terms of both DNA and RNA soil microbial communities from the Long-term Assessment of Miscanthus Productivity and Sustainability (LAMPS) field trial. DNA and RNA were extracted from 271 samples, and 16S small subunit (SSU) rRNA amplicon sequencing was performed to characterize microbial community structure. Significant differences were observed in the resulting soil microbiomes and were best explained by the sequencing library of origin, either DNA or RNA. Similar numbers of membership were detected in DNA and RNA microbial communities, with more than 90% of membership shared. However, the profile of dominant membership within DNA and RNA differed, with varying proportions of Actinobacteria and Proteobacteria and Firmicutes and Proteobacteria. Only RNA microbial communities showed seasonal responses to nitrogen fertilization, and these differences were associated with nitrogen-cycling bacteria. The relative abundance of bacteria associated with nitrogen cycling was 7-fold higher in RNA than in DNA, and genes associated with denitrifying bacteria were significantly enriched in RNA, suggesting that these bacteria may be underestimated with DNA-only approaches. Our findings indicate that RNA-based SSU characterization can be a significant and complementing resource for understanding the role of soil microbiomes in bioenergy crop production. IMPORTANCEMiscanthus × giganteus is a promising candidate for bioeconomy cropping systems; however, it remains unclear how the soil microbiome supplies nitrogen to this low-input crop. DNA-based techniques are used to provide community characterization, but may miss important metabolically active taxa. By analyzing both DNA- and actively transcribed RNA-based microbial communities, we found that nitrogen cycling taxa in the soil microbiome may be underestimated using only DNA-based approaches. Accurately understanding the role of microbes and how they cycle nutrients is important for the development of sustainable bioenergy crops, and RNA-based approaches are recommended as a complement to DNA approaches to better understand the microbial, plant, and management interactions.

Dataset Information

Cadmium and Mercury phytostabilization from soil using Miscanthus × giganteus.

Publications

Cadmium and Mercury phytostabilization from soil using Miscanthus × giganteus.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets