Dataset Information

Loss of Arabidopsis ?-COP Function Affects Golgi Structure, Plant Growth and Tolerance to Salt Stress.

ABSTRACT: The early secretory pathway involves bidirectional transport between the endoplasmic reticulum (ER) and the Golgi apparatus and is mediated by coat protein complex I (COPI)-coated and coat protein complex II (COPII)-coated vesicles. COPII vesicles are involved in ER to Golgi transport meanwhile COPI vesicles mediate intra-Golgi transport and retrograde transport from the Golgi apparatus to the ER. The key component of COPI vesicles is the coatomer complex, that is composed of seven subunits (?/?/?'/?/?/?/?). In Arabidopsis two genes coding for the ?-COP subunit have been identified, which are the result of recent tandem duplication. Here we have used a loss-of-function approach to study the function of ?-COP. The results we have obtained suggest that ?-COP is required for plant growth and salt tolerance. In addition, ?-COP function seems to be required for maintaining the structure of the Golgi apparatus.

SUBMITTER: Sanchez-Simarro J

PROVIDER: S-EPMC7175232 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Loss of <i>Arabidopsis β-COP</i> Function Affects Golgi Structure, Plant Growth and Tolerance to Salt Stress.

Sánchez-Simarro Judit J Bernat-Silvestre César C Gimeno-Ferrer Fátima F Selvi-Martínez Pilar P Montero-Pau Javier J Aniento Fernando F Marcote María Jesús MJ

Frontiers in plant science 20200415

The early secretory pathway involves bidirectional transport between the endoplasmic reticulum (ER) and the Golgi apparatus and is mediated by coat protein complex I (COPI)-coated and coat protein complex II (COPII)-coated vesicles. COPII vesicles are involved in ER to Golgi transport meanwhile COPI vesicles mediate intra-Golgi transport and retrograde transport from the Golgi apparatus to the ER. The key component of COPI vesicles is the coatomer complex, that is composed of seven subunits (α/β ...[more]

PMID: 32351533

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Project description:Plant growth promoting rhizobacteria (PGPR) are a functionally diverse group of microbes having immense potential as biostimulants and stress alleviators. Their exploitation in agro-ecosystems as an eco-friendly and cost-effective alternative to traditional chemical inputs may positively affect agricultural productivity and environmental sustainability. The present study describes selected rhizobacteria, from a range of origins, having plant growth promoting potential under controlled conditions. A total of 98 isolates (ectophytic or endophytic) from various crop and uncultivated plants were screened, out of which four endophytes (n, L, K and Y) from Phalaris arundinacea, Solanum dulcamara, Scorzoneroides autumnalis, and Glycine max, respectively, were selected in vitro for their vegetative growth stimulating effects on Arabidopsis thaliana Col-0 seedlings with regard to leaf surface area and shoot fresh weight. A 16S rRNA gene sequencing analysis of the strains indicated that these isolates belong to the genera Pseudomonas, Bacillus, Mucilaginibacter and Rhizobium. Strains were then further tested for their effects on abiotic stress alleviation under both Petri-plate and pot conditions. Results from Petri-dish assay indicated strains L, K and Y alleviated salt stress in Arabidopsis seedlings, while strains K and Y conferred increases in fresh weight and leaf area under osmotic stress. Results from subsequent in vivo trials indicated all the isolates, especially strains L, K and Y, distinctly increased A. thaliana growth under both normal and high salinity conditions, as compared to control plants. The activity of antioxidant enzymes (ascorbate peroxidase, catalase and peroxidase), proline content and total antioxidative capacity also differed in the inoculated A. thaliana plants. Furthermore, a study on spatial distribution of the four strains, using either conventional Petri-plate counts or GFP-tagged bacteria, indicated that all four strains were able to colonize the endosphere of A. thaliana root tissue. Thus, the study revealed that the four selected rhizobacteria are good candidates to be explored as plant growth stimulators, which also possess salt stress mitigating property, partially by regulating osmolytes and antioxidant enzymes. Moreover, the study is the first report of Scorzoneroides autumnalis (fall dandelion) and Solanum dulcamara (bittersweet) associated endophytes with PGP effects.

Dataset Information

Loss of Arabidopsis ?-COP Function Affects Golgi Structure, Plant Growth and Tolerance to Salt Stress.

Publications

Loss of <i>Arabidopsis β-COP</i> Function Affects Golgi Structure, Plant Growth and Tolerance to Salt Stress.

Similar Datasets

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