Ontology highlight
ABSTRACT:
SUBMITTER: Gupta A
PROVIDER: S-EPMC7176048 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Gupta Aarushi A Rivera-Molina Felix F Xi Zhiqun Z Toomre Derek D Schepartz Alanna A
Nature chemical biology 20200224 4
We report new lipid-based, high-density, environmentally sensitive (HIDE) probes that accurately and selectively image endo-lysosomes and their dynamics at super-resolution for extended times. Treatment of live cells with the small molecules DiIC<sub>16</sub>TCO or DiIC<sub>16'</sub>TCO followed by in situ tetrazine ligation reaction with the silicon-rhodamine dye SiR-Tz generates the HIDE probes DiIC<sub>16</sub>-SiR and DiIC<sub>16'</sub>-SiR in the endo-lysosomal membrane. These new probes su ...[more]