Ontology highlight
ABSTRACT:
SUBMITTER: Wang F
PROVIDER: S-EPMC7176847 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Wang Feiran F Tang Chong C Xu Dong D Tang Yijie Y Jiang Yasu Y Gao Xuesong X Xu Junfei J
Journal of cellular and molecular medicine 20200311 8
Nowadays, research on CSCs is still in an initial stage, and there are few studies reporting the successful isolation and identification of CSCs. In the present study, we attempted to isolate CSCs through cultivating the cell line MKN45 in defined serum-free medium and study the expression of stem cell markers or related proteins (Oct3/4, Sox2, Nanog and CD44) in CSCs. Moreover, immunofluorescence staining was performed to validate the stem cell markers of spheroid body-forming cells. Further ex ...[more]