Dataset Information

Identification of QTLs associated with curd architecture in cauliflower.

ABSTRACT: BACKGROUND:Curd architecture is one of the most important characters determining the curd morphology of cauliflower. However, the genetic mechanism dissection of this complex trait at molecular level is lacking. Genes/QTLs responsible for the morphological differences between present-day loose-curd and compact-curd cauliflower haven't been well revealed. RESULTS:Herein, by using a common compact-curd parent and two loose-curd parents, we developed two double haploid (DH) populations including 122 and 79 lines, respectively. For each population, we decomposed the curd architecture concept into four parameters (basal diameter, stalk length, stalk angle and curd solidity), and collected corresponding phenotypic data for each parameter across two environments. The Kosambi function and composite interval mapping algorithm were conducted to construct the linkage map and analyze the QTLs associated with curd architecture parameters. A total of 20 QTLs were detected with the minimum likelihood of odd (LOD) values ranging from 2.61 to 8.38 and the percentage of the phenotypic variance explained by each QTL (PVE) varying between 7.69 and 25.10%. Of these, two QTLs controlling stalk length (qSL.C6-1, qSL.C6-2) and two QTLs controlling curd solidity (qCS.C6-1 and qCS.C6-2) were steadily expressed in both environments. Further, qSL.C6-1, qSL.C6-2, qCS.C6-1 and qCS.C6-4 fell into the same chromosomal region of the reference genome, indicating that these loci are involved in pleiotropic effects or are tightly linked. CONCLUSION:The current study identified a series of QTLs associated with curd architecture parameters, which might contribute essentially to the formation of present-day loose-curd cauliflower that is widely cultivated in China. These results may pave the way for intensive deciphering the molecular mechanisms of curd development and for marker-assisted selection of curd morphology in cauliflower breeding.

SUBMITTER: Zhao ZQ

PROVIDER: S-EPMC7178959 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Identification of QTLs associated with curd architecture in cauliflower.

Zhao Zhen-Qing ZQ Sheng Xiao-Guang XG Yu Hui-Fang HF Wang Jian-Sheng JS Shen Yu-Sen YS Gu Hong-Hui HH

BMC plant biology 20200422 1

<h4>Background</h4>Curd architecture is one of the most important characters determining the curd morphology of cauliflower. However, the genetic mechanism dissection of this complex trait at molecular level is lacking. Genes/QTLs responsible for the morphological differences between present-day loose-curd and compact-curd cauliflower haven't been well revealed.<h4>Results</h4>Herein, by using a common compact-curd parent and two loose-curd parents, we developed two double haploid (DH) populatio ...[more]

PMID: 32321422

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Project description:The present study was undertaken to know the genetics of purple color of cauliflower curds using a Sicilian purple 'PC-1' and a white curding mid-late group genotype of Indian cauliflower. For this, a cross was attempted between 'DC-466' (white curd) and 'PC-1' (purple curd) and observed intermediate level of purple pigmentation on curds in F1 plants. Segregation of F2 population (173) revealed that the purple color of the curd was governed by a single gene dominant over white, but the expression of trait was incomplete. It was substantiated by segregation of plants of BC1 and F2:3(intermediate) generations into 1(white):1(intermediate) and 1(white):2(intermediate):1(intense), respectively. The F2, B1, and B2 generations segregated into purple(intermediate to intense): white curding plants in the ratio of 126: 47, 26:24, and 40:0, respectively fitting well with the Mendelian ratio of single gene for purple curds. However, purple pigmentation on curds ranged from very light to intense, which corroborated with the wide range of anthocyanin content in F2 (3.81-48.21 mg/100 g fw). Out of three molecular markers from high resolution map of Pr gene in purple color cauliflower 'Graffiti', only BoMYB3 marker could distinguish purple and white curding parents but did not show co-segregation while investigated in F2 population. Expression of BoMYB1 gene was up regulated in both the purple curd genotypes 'PC-1' and 'Graffiti' in comparison to white curded 'DC-466', while BoMYB2 gene was slightly upregulated in 'PC-1' but down regulated in 'Graffiti'. Occurrence of 'broccoli type' F2 individuals and their genetic stability in F2:3 support the intermediate position of 'Sicilian purple' between broccoli (Calabrese) and cauliflower. There was not any correlation between curd coloration and pigmentation on apical leaf and stem portion, indicating difference of expression in 'PC-1' than 'Graffiti'. The information obtained is useful for breeding anthocyanin rich attractive purple curding 'specialty cauliflower' for better consumer health and growers' earnings.

Dataset Information

Identification of QTLs associated with curd architecture in cauliflower.

Publications

Identification of QTLs associated with curd architecture in cauliflower.

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