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Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO3) Responsiveness and ?-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus.

ABSTRACT: Addition of sodium bicarbonate (NaHCO3) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to ?-lactams. We investigated the prevalence of this phenotype (NaHCO3 responsiveness) to two ?-lactams among 58 clinical MRSA bloodstream isolates. Of note, ?75% and ?36% of isolates displayed the NaHCO3 responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic ?-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO3 responsiveness for OXA.

SUBMITTER: Ersoy SC 

PROVIDER: S-EPMC7179597 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO<sub>3</sub>) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus.

Ersoy Selvi C SC   Otmishi Mariam M   Milan Vanessa T VT   Li Liang L   Pak Youngju Y   Mediavilla Jose J   Chen Liang L   Kreiswirth Barry B   Chambers Henry F HF   Proctor Richard A RA   Xiong Yan Q YQ   Fowler Vance G VG   Bayer Arnold S AS  

Antimicrobial agents and chemotherapy 20200421 5

Addition of sodium bicarbonate (NaHCO<sub>3</sub>) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO<sub>3</sub> responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO<sub>3</sub> responsiveness phenotype to cefazolin (CFZ) and oxacillin (  ...[more]

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