Ultralow-frequency neural entrainment to pain.
Ontology highlight
ABSTRACT:
SUBMITTER: Guo Y
PROVIDER: S-EPMC7179945 | biostudies-literature |
REPOSITORIES: biostudies-literature
Similar Datasets
Project description:We propose a modular architecture for neuromorphic closed-loop control based on bistable relaxation oscillator modules consisting of three spiking neurons each. Like its biological prototypes, this basic component is robust to parameter variation but can be modulated by external inputs. By combining these modules, we can construct a neural state machine capable of generating the cyclic or repetitive behaviors necessary for legged locomotion. A concrete case study for the approach is provided by a modular robot constructed from flexible plastic volumetric pixels, in which we produce a forward crawling gait entrained to the natural frequency of the robot by a minimal system of twelve neurons organized into four modules.
| S-EPMC7577446 | biostudies-literature
Project description:Duplex Sequencing (DS) is a next-generation sequencing methodology capable of detecting a single mutation among >1 × 10(7) wild-type nucleotides, thereby enabling the study of heterogeneous populations and very-low-frequency genetic alterations. DS can be applied to any double-stranded DNA sample, but it is ideal for small genomic regions of <1 Mb in size. The method relies on the ligation of sequencing adapters harboring random yet complementary double-stranded nucleotide sequences to the sample DNA of interest. Individually labeled strands are then PCR-amplified, creating sequence 'families' that share a common tag sequence derived from the two original complementary strands. Mutations are scored only if the variant is present in the PCR families arising from both of the two DNA strands. Here we provide a detailed protocol for efficient DS adapter synthesis, library preparation and target enrichment, as well as an overview of the data analysis workflow. The protocol typically takes 1-3 d.
| S-EPMC4271547 | biostudies-literature
Project description:A controversy exists on photic driving in the human visual cortex evoked by intermittent photic stimulation. Frequency entrainment and resonance phenomena are reported for frequencies higher than 12 Hz in some studies while missing in others. We hypothesized that this might be due to different experimental conditions, since both high and low intensity light stimulation were used. However, most studies do not report radiometric measurements, which makes it impossible to categorize the stimulation according to photopic, mesopic, and scotopic vision. Low intensity light stimulation might lead to scotopic vision, where rod perception dominates. In this study, we investigated photic driving for rod-dominated visual input under scotopic conditions. Twelve healthy volunteers were stimulated with low intensity light flashes at 20 stimulation frequencies, leading to rod activation only. The frequencies were multiples of the individual alpha frequency (?) of each volunteer in the range from 0.40 to 2.30(?)?. Three hundred and six-channel whole head magnetoencephalography recordings were analyzed in time, frequency, and spatiotemporal domains with the Topographic Matching Pursuit algorithm. We found resonance phenomena and frequency entrainment for stimulations at or close to the individual alpha frequency (0.90-1.10(?)?) and half of the alpha frequency (0.40-0.55(?)?). No signs of resonance and frequency entrainment phenomena were revealed around 2.00(?)?. Instead, on-responses at the beginning and off-responses at the end of each stimulation train were observed for the first time in a photic driving experiment at frequencies of 1.30-2.30(?)?, indicating that the flicker fusion threshold was reached. All results, the resonance and entrainment as well as the fusion effects, provide evidence for rod-dominated photic driving in the visual cortex.
| S-EPMC4989477 | biostudies-literature
Project description:A high-speed micromotor is usually actuated by a power source with high voltage and frequency. Here we report a triboelectric micromotor by coupling a micromotor and a triboelectric nanogenerator, in which the micromotor can be actuated by ultralow-frequency mechanical stimuli. The performances of the triboelectric micromotor are exhibited at various structural parameters of the micromotor, as well as at different mechanical stimuli of the triboelectric nanogenerator. With a sliding range of 50 mm at 0.1 Hz, the micromotor can start to rotate and reach over 1000 r min-1 at 0.8 Hz. The maximum operation efficiency of the triboelectric micromotor can reach 41%. Additionally, the micromotor is demonstrated in two scanning systems for information recognition. This work has realized a high-speed micromotor actuated by ultralow frequency mechanical stimuli without an external power supply, which has extended the application of triboelectric nanogenerator in micro/nano electromechanical systems, intelligent robots and autonomous driving.
| S-EPMC6534612 | biostudies-literature
Project description:Striatal fast-spiking interneurons (FSIs) fire in variable-length runs of action potentials at 20-200 spikes/s separated by pauses. In vivo, or with fluctuating applied current, both runs and pauses become briefer and more variable. During runs, spikes are entrained specifically to gamma-frequency components of the input fluctuations. We stimulated parvalbumin-expressing striatal FSIs in mouse brain slices with broadband noise currents added to direct current steps and measured spike entrainment across all frequencies. As the constant current level was increased, FSIs produced longer runs and showed sharper frequency tuning, with best entrainment at the stimulus frequency matching their intrarun firing rate. We separated the contributions of previous spikes from that of the fluctuating stimulus, revealing a strong contribution of previous action potentials to gamma-frequency entrainment. In contrast, after subtraction of the effect inherited from the previous spike, the remaining stimulus contribution to spike generation was less sharply tuned, showing a larger contribution of lower frequencies. The frequency specificity of entrainment within a run was reproduced with a phase resetting model based on experimentally measured phase resetting curves of the same FSIs. In the model, broadly tuned phase entrainment for the first spike in a run evolved into sharply tuned gamma entrainment over the next few spikes. The data and modeling results indicate that for FSIs firing in brief runs and pauses firing within runs is entrained by gamma-frequency components of the input, whereas the onset timing of runs may be sensitive to a wider range of stimulus frequency components.NEW & NOTEWORTHY Specific types of neurons entrain their spikes to particular oscillation frequencies in their synaptic input. This entrainment is commonly understood in terms of the subthreshold voltage response, but how this translates to spiking is not clear. We show that in striatal fast-spiking interneurons, entrainment to gamma-frequency input depends on rhythmic spike runs and is explained by the phase resetting curve, whereas run initiation can be triggered by a broad range of input frequencies.
| S-EPMC6766739 | biostudies-literature
Project description:A method of reconstructing perceived or imagined music by analyzing brain activity has not yet been established. As a first step toward developing such a method, we aimed to reconstruct the imagery of rhythm, which is one element of music. It has been reported that a periodic electroencephalogram (EEG) response is elicited while a human imagines a binary or ternary meter on a musical beat. However, it is not clear whether or not brain activity synchronizes with fully imagined beat and meter without auditory stimuli. To investigate neural entrainment to imagined rhythm during auditory imagery of beat and meter, we recorded EEG while nine participants (eight males and one female) imagined three types of rhythm without auditory stimuli but with visual timing, and then we analyzed the amplitude spectra of the EEG. We also recorded EEG while the participants only gazed at the visual timing as a control condition to confirm the visual effect. Furthermore, we derived features of the EEG using canonical correlation analysis (CCA) and conducted an experiment to individually classify the three types of imagined rhythm from the EEG. The results showed that classification accuracies exceeded the chance level in all participants. These results suggest that auditory imagery of meter elicits a periodic EEG response that changes at the imagined beat and meter frequency even in the fully imagined conditions. This study represents the first step toward the realization of a method for reconstructing the imagined music from brain activity.
| S-EPMC5645537 | biostudies-literature
Project description:Sensitivity of mechanical detection by the inner ear is dependent upon a highly nonlinear response to the applied stimulus. Here we show that a system of differential equations that support a subcritical Hopf bifurcation, with a feedback mechanism that tunes an internal control parameter, captures a wide range of experimental results. The proposed model reproduces the regime in which spontaneous hair bundle oscillations are bistable, with sporadic transitions between the oscillatory and the quiescent state. Furthermore, it is shown, both experimentally and theoretically, that the application of a high-amplitude stimulus to the bistable system can temporarily render it quiescent before recovery of the limit cycle oscillations. Finally, we demonstrate that the application of low-amplitude stimuli can entrain bundle motility either by mode-locking to the spontaneous oscillation or by mode-locking the transition between the quiescent and oscillatory states.
| S-EPMC3628560 | biostudies-literature
Project description:Changes in gamma oscillations (20-50?Hz) have been observed in several neurological disorders. However, the relationship between gamma oscillations and cellular pathologies is unclear. Here we show reduced, behaviourally driven gamma oscillations before the onset of plaque formation or cognitive decline in a mouse model of Alzheimer's disease. Optogenetically driving fast-spiking parvalbumin-positive (FS-PV)-interneurons at gamma (40?Hz), but not other frequencies, reduces levels of amyloid-? (A?)1-40 and A? 1-42 isoforms. Gene expression profiling revealed induction of genes associated with morphological transformation of microglia, and histological analysis confirmed increased microglia co-localization with A?. Subsequently, we designed a non-invasive 40?Hz light-flickering regime that reduced A?1-40 and A?1-42 levels in the visual cortex of pre-depositing mice and mitigated plaque load in aged, depositing mice. Our findings uncover a previously unappreciated function of gamma rhythms in recruiting both neuronal and glial responses to attenuate Alzheimer's-disease-associated pathology.
| S-EPMC5656389 | biostudies-literature
Project description:The formation of excitons in OLEDs is spin dependent and can be controlled by electron-paramagnetic resonance, affecting device resistance and electroluminescence yield. We explore electrically detected magnetic resonance in the regime of very low magnetic fields (<1?mT). A pronounced feature emerges at zero field in addition to the conventional spin- 1/2 Zeeman resonance for which the Larmor frequency matches that of the incident radiation. By comparing a conventional ?-conjugated polymer as the active material to a perdeuterated analogue, we demonstrate the interplay between the zero-field feature and local hyperfine fields. The zero-field peak results from a quasistatic magnetic-field effect of the RF radiation for periods comparable to the carrier-pair lifetime. Zeeman resonances are resolved down to 3.2?MHz, approximately twice the Larmor frequency of an electron in Earth's field. However, since reducing hyperfine fields sharpens the Zeeman peak at the cost of an increased zero-field peak, we suggest that this result may constitute a fundamental low-field limit of magnetic resonance in carrier-pair-based systems. OLEDs offer an alternative solid-state platform to investigate the radical-pair mechanism of magnetic-field effects in photochemical reactions, allowing models of biological magnetoreception to be tested by measuring spin decoherence directly in the time domain by pulsed experiments.
| S-EPMC7317727 | biostudies-literature
Project description:OBJECTIVE:Optogenetic modulation of neural activity is a ubiquitous tool for basic investigation of brain circuits. While the majority of optogenetic paradigms rely on short light pulses to evoke synchronized activity of optically sensitized cells, many neurobiological processes are associated with slow local field potential (LFP) oscillations. Therefore, we developed a hybrid fiber probe capable of simultaneous electrophysiological recording and optical stimulation and used it to investigate the utility of sinusoidal light stimulation for evoking oscillatory neural activity in vivo across a broad frequency range. APPROACH:We fabricated hybrid fiber probes comprising a hollow cylindrical array of 9 electrodes and a flexible optical waveguide integrated within the core. We implanted these probes in the hippocampus of transgenic Thy1-ChR2-YFP mice that broadly express the blue-light sensitive cation channel channelrhodopsin 2 (ChR2) in excitatory neurons across the brain. The effects of the sinusoidal light stimulation were characterized and contrasted with those corresponding to pulsed stimulation in the frequency range of physiological LFP rhythms (3-128 Hz). MAIN RESULTS:Within hybrid probes, metal electrode surfaces were vertically aligned with the waveguide tip, which minimized optical stimulation artifacts in neurophysiological recordings. Sinusoidal stimulation resulted in reliable and coherent entrainment of LFP oscillations up to 70 Hz, the cutoff frequency of ChR2, with response amplitudes inversely scaling with the stimulation frequencies. Effectiveness of the stimulation was maintained for two months following implantation. SIGNIFICANCE:Alternative stimulation patterns complementing existing pulsed protocols, in particular sinusoidal light stimulation, are a prerequisite for investigating the physiological mechanisms underlying brain rhythms. So far, studies applying sinusoidal stimulation in vivo were limited to single stimulation frequencies. We show the feasibility of sinusoidal stimulation in vivo to induce coherent LFP oscillations across the entire frequency spectrum supported by the gating dynamics of ChR2 and introduce a hybrid fiber probe tailored to continuous light stimulation.
| S-EPMC6125198 | biostudies-other