One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway.
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ABSTRACT:
SUBMITTER: Mukherjee A
PROVIDER: S-EPMC7179975 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Publications
One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway.
Mukherjee Abhirup A Stathos Mark E ME Varner Chad C Arsiwala Ammar A Frey Steven S Hu Yuge Y Smalley David M DM Schaffer David V DV Kane Ravi S RS
Chemical communications (Cambridge, England) 20200301 25
Fragment antigen-binding domains (Fabs) from anti-Frizzled and anti-LRP6 monoclonal antibodies were conjugated using SpyTag-SpyCatcher chemistry via a one-pot reaction. The resulting synthetic heterodimeric agonist outperformed the natural ligand, Wnt-3a, in activating canonical Wnt signaling in mammalian cells. This approach should be broadly applicable to activate receptor-mediated cellular signaling. ...[more]
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