ASIC1a channels regulate mitochondrial ion signaling and energy homeostasis in neurons.

ABSTRACT: Acid-sensing ion channel 1a (ASIC1a) is well-known to play a major pathophysiological role during brain ischemia linked to acute acidosis of ~pH 6, whereas its function during physiological brain activity, linked to much milder pH changes, is still poorly understood. Here, by performing live cell imaging utilizing Na⁺ and Ca²⁺ sensitive and spatially specific fluorescent dyes, we investigated the role of ASIC1a in cytosolic Na⁺ and Ca²⁺ signals elicited by a mild extracellular drop from pH 7.4 to 7.0 and how these affect mitochondrial Na⁺ and Ca²⁺ signaling or metabolic activity. We show that in mouse primary cortical neurons, this small extracellular pH change triggers cytosolic Na⁺ and Ca²⁺ waves that

SUBMITTER: Savic Azoulay I 

PROVIDER: S-EPMC7180113 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature