Dataset Information

Aggregation and Molecular Properties of ?-Glucosidase Isoform II in Chayote (Sechium edule).

ABSTRACT: The presence of isoforms of ?-glucosidase has been reported in some grasses such as sorghum, rice and maize. This work aims to extract and characterize isoform II in ?-glucosidase from S. edule. A crude extract was prepared without buffer solution and adjusted to pH 4.6. Contaminating proteins were precipitated at 4 °C for 24 h. The supernatant was purified by chromatography on carboxymethyl cellulose (CMC) column, molecular exclusion on Sephacryl S-200HR, and exchange anionic on QFF column. Electrophoretic analyzes revealed a purified enzyme with aggregating molecular complex on SDS-PAGE, Native-PAGE, and AU-PAGE. Twelve peptides fragments were identified by nano liquid chromatography-tandem mass spectrometry (nano LC-ESI-MS/MS), which presented as 61% identical to Cucurbita moschata ?-glucosidase and 55.74% identical to ?-glucosidase from Cucumis sativus, another Cucurbitaceous member. The relative masses which contained 39% hydrophobic amino acids ranged from 982.49 to 2,781.26. The enzyme showed a specificity to ?-d-glucose with a Km of 4.59 mM, a Vmax value of 104.3 ?M?min-1 and a kcat of 10,087 ?M?min-1 using p-nitrophenyl-?-D-glucopyranoside. The presence of molecular aggregates can be attributed to non-polar amino acids. This property is not mediated by a ?-glucosidase aggregating factor (BGAF) as in grasses (maize and sorghum). The role of these aggregates is discussed.

SUBMITTER: Cruz Rodriguez A

PROVIDER: S-EPMC7180739 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Publications

Aggregation and Molecular Properties of β-Glucosidase Isoform II in Chayote (<i>Sechium edule</i>).

Cruz Rodríguez Alberto A Sánchez Esperanza Fabiola Anaid FA Pérez-Campos Eduardo E Hernández-Huerta María Teresa MT Pérez-Campos Mayoral Laura L Matias-Cervantes Carlos Alberto CA Martínez Barras Alexis A Mayoral-Andrade Gabriel G Santos Pineda Luis Ángel LÁ Díaz Barrita Aymara Judith AJ Zenteno Edgar E Romero Díaz Carlos C Martínez Cruz Ruth R Pérez-Campos Mayoral Eduardo E Bernabé Pérez Edith Alhelí EA Pérez Santiago Alma Dolores AD Pina-Canseco María Del Socorro MDS Martínez Cruz Margarito M

Molecules (Basel, Switzerland) 20200408 7

The presence of isoforms of β-glucosidase has been reported in some grasses such as sorghum, rice and maize. This work aims to extract and characterize isoform II in β-glucosidase from <i>S. edule</i>. A crude extract was prepared without buffer solution and adjusted to pH 4.6. Contaminating proteins were precipitated at 4 °C for 24 h. The supernatant was purified by chromatography on carboxymethyl cellulose (CMC) column, molecular exclusion on Sephacryl S-200HR, and exchange anionic on QFF colu ...[more]

PMID: 32276317

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Project description:BackgroundChayote is an underutilized species of Cucurbitaceae. It is rich in nutrients such as protein, minerals, phenols and its extracts have anti-cardiovascular and anti-cancer effects, making it a versatile plant for both medicinal and culinary purposes. Although research on its root tuber is limited, they are rich in starch and have a structure similar to that of potatoes, cassava, and sweet potatoes. Therefore, they can serve as potential substitutes for potatoes and offer promising prospects as agricultural and industrial resources. However, the physiological and cellular mechanisms of chayote root tuber formation and development are still unclear.ResultsIn this study, we observed the growth habit of 'Tuershao' (high yield of root tuber). The results revealed that the tuber enlargement period of 'Tuershao' lasts approximately 120 days, with the early enlargement phase occurring during 0-30 days, rapid enlargement phase during 30-90 days, and maturation phase during 90-120 days. Physiological indicators demonstrated a gradual increase in starch content as the tuber developed. The activities of sucrose synthase (SUS) and invertase (VIN) showed a consistent trend, reaching the highest level in the rapid expansion period, which was the key enzyme affecting tuber expansion. Moreover, the special petal like structure formed by the secondary phloem and secondary xylem of the tuber resulted in its enlargement, facilitating the accumulation of abundant starch within the thin-walled cells of this structure. Principal component analysis further confirmed that starch content, SUS and VIN activities, as well as the concentrations of calcium (Ca), iron (Fe), and selenium (Se), were the major factors influencing tuber development. Moreover, the low temperature environment not only promoted the growth of 'Tuershao' tubers but also enhanced the accumulation of nutritional substances.ConclusionsThese findings contribute to a deeper understanding of the formation and developmental mechanisms of 'Tuershao' tubers, providing valuable guidance for cultivation practices aimed at improving crop yield.

Dataset Information

Aggregation and Molecular Properties of ?-Glucosidase Isoform II in Chayote (Sechium edule).

Publications

Aggregation and Molecular Properties of β-Glucosidase Isoform II in Chayote (<i>Sechium edule</i>).

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