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Rifamycin O, An Alternative Anti-Mycobacterium abscessus Agent.

ABSTRACT: Mycobacterium abscessus is the most difficult-to-treat nontuberculous mycobacteria because of its resistance to many antibiotics. In this study, we screened the Korea Chemical Bank library for a bioluminescent reporter assay to identify molecules capable of acting against M. abscessus. On application of the assay, rifamycin O showed excellent in vitro activity with a narrow range of the minimum inhibitory concentration required to inhibit the growth of 90% of the bacterium (MIC₉₀ = 4.0-6.2 ?M); its in vivo efficacy in the zebrafish (Danio rerio) infection model was comparable to that of rifabutin at 25 ?M. Furthermore, rifamycin O did not show significant toxicity in cells and the zebrafish model. These results are the first in vivo indication that rifamycin O may be a drug candidate for treating M. abscessus infections.

SUBMITTER: Hanh BTB

PROVIDER: S-EPMC7181020 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Rifamycin O, An Alternative Anti-Mycobacterium abscessus Agent.

Hanh Bui Thi Bich BTB Park June-Woo JW Kim Tae Ho TH Kim Jae-Sung JS Yang Chul-Su CS Jang Kiseok K Cui Jinsheng J Oh Dong-Chan DC Jang Jichan J

Molecules (Basel, Switzerland) 20200331 7

Mycobacterium abscessus is the most difficult-to-treat nontuberculous mycobacteria because of its resistance to many antibiotics. In this study, we screened the Korea Chemical Bank library for a bioluminescent reporter assay to identify molecules capable of acting against M. abscessus. On application of the assay, rifamycin O showed excellent in vitro activity with a narrow range of the minimum inhibitory concentration required to inhibit the growth of 90% of the bacterium (MIC<sub ...[more]

PMID: 32244387

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