Ontology highlight
ABSTRACT:
SUBMITTER: Chang CF
PROVIDER: S-EPMC7181027 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Chang Chun-Fang CF Hamase Kenji K Tsunoda Makoto M
Molecules (Basel, Switzerland) 20200409 7
Homocysteine and related thiols (cysteine, cysteinylglycine, and glutathione) in the urine of a cystathionine β-synthase (CBS)-deficient mouse model were quantified using hydrophilic interaction chromatography with fluorescence detection. Urine samples were incubated with tris(2-carboxyethyl) phosphine to reduce disulfide bonds into thiols. After deproteinization, thiols were fluorescently derivatized with ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F). Homocysteine, cysteine, cystei ...[more]