Lipid-coated ZnO nanoparticles synthesis, characterization and cytotoxicity studies in cancer cell.

ABSTRACT: ZnO nanoparticles are widely used in biological, chemical, and medical fields, but their toxicity impedes their wide application. In this study, pristine ZnO NPs (~?7 nm;?~?18 nm;?~?49 nm) and lipid-coated ZnO NPs (~?13 nm;?~?22 nm;?~?52 nm) with different morphologies were prepared by chemical method and characterized by TEM, XRD, HRTEM, FTIR, and DLS. Our results showed that the lipid-coated ZnO NPs (~?13 nm;?~?22 nm;?~?52 nm) groups improved the colloidal stability, prevented the aggregation and dissolution of nanocrystal particles in the solution, inhibited the dissolution of ZnO NPs into Zn²⁺ cations, and reduced cytotoxicity more efficiently than the pristine ZnO NPs (~?7 nm;?~?18 nm;?~?49 nm). Compared to the lipid-coated ZnO NPs, pristine ZnO NPs (~?7 nm;?~?18 nm;?~?49 nm) could dose-dependently destroy the cells at low concentrations. At the same concentration, ZnO NPs (~?7 nm) exhibited the highest cytotoxicity. These results could provide a basis for the toxicological study of the nanoparticles and direct future investigations for preventing strong aggregation, reducing the toxic effects of lipid-bilayer and promoting the uptake of nanoparticles by HeLa cells efficiently.

SUBMITTER: Cao D 

PROVIDER: S-EPMC7181468 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature