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Detection of the Asian II genotype of dengue virus serotype 2 in humans and mosquitoes in Brazil.

ABSTRACT: INTRODUCTION:DENV-2 is the cause of most dengue epidemics worldwide and is associated with severe cases. METHODS:We investigated arboviruses in 164 serum samples collected from patients presenting with clinical symptoms of dengue fever and 152 mosquito pools. RESULTS:We detected the Asian II genotype of DENV-2 in humans and mosquitoes. Our results confirmed the circulation of the Asian II genotype in Brazil, in addition to the prevalent Asian/American genotype. CONCLUSIONS:The detection of Asian II genotype of DENV-2 in mosquito pools collected in a forest park may be related to a spillback event of human dengue virus.

SUBMITTER: Kubiszeski JR

PROVIDER: S-EPMC7182292 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Detection of the Asian II genotype of dengue virus serotype 2 in humans and mosquitoes in Brazil.

Kubiszeski Janaina Rigotti JR Vieira Carla Julia da Silva Pessoa CJDSP Thies Sirlei Franck SF Silva David José Ferreira da DJFD Barreto Eriana Serpa ES Mondini Adriano A Bronzoni Roberta Vieria de Morais RVM

Revista da Sociedade Brasileira de Medicina Tropical 20200422

<h4>Introduction</h4>DENV-2 is the cause of most dengue epidemics worldwide and is associated with severe cases.<h4>Methods</h4>We investigated arboviruses in 164 serum samples collected from patients presenting with clinical symptoms of dengue fever and 152 mosquito pools.<h4>Results</h4>We detected the Asian II genotype of DENV-2 in humans and mosquitoes. Our results confirmed the circulation of the Asian II genotype in Brazil, in addition to the prevalent Asian/American genotype.<h4>Conclusio ...[more]

PMID: 32321091

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Project description:BACKGROUND: Dengue, a mosquito-borne viral infection caused by one of the four dengue virus (DENV) serotypes (DENV-1 to 4), replicate alternately on the mosquito vector and human host and are responsible for infections throughout tropical and subtropical regions of the world. In Brazil, the disease has become a major public health problem and the introduction of DENV-3 in 2000 in Rio de Janeiro (RJ) was associated with severe dengue epidemics. The potential emergence of strains associated with severe disease highlights the need for the surveillance of DENV in human host and vectors. METHODS: Aiming to contribute for DENV phylogenetic and vector-virus-human host studies, we sequenced the entire genome of one DENV-3 isolated from naturally infected Aedes aegypti from RJ in 2001 and characterized the 3' UTR from strains isolated from mosquitoes and humans. Mosquitoes were pooled and submitted to virus isolation in Ae. albopictus C6/36 cells and the infecting serotype was identified by immunofluorescence using type-specific monoclonal antibody. Sequence analysis was performed using BioEdit software, the multiple alignments were performed using CLUSTAL W and the phylogenetic analysis by MEGA 5, using the Neighbor-joining method. Secondary structure prediction was performed by using the MFOLD program. RESULTS: Exclusive substitutions and a substitution leading to a stop codon on the NS5 gene were observed in the DENV-3 isolated from a naturally infected Ae. aegypti and fully sequenced. As an 8- nucleotides deletion was observed within the 11- nucleotides (nts) insertion on the variable region (VR) from the 3'UTR in this isolate, we further sequenced other DENV-3 from both mosquitoes and humans. The majority of DENV-3 from RJ analyzed were characterized by the 11-nts insertion in the VR of the 3'UTR, despite the observation of strains carrying the 8-nts deletion. The latter presented similar secondary structures, however not all strains presenting the 11-nts insertion were similar in the predicted secondary structure. CONCLUSIONS: The phylogeny based on the analysis of the complete genome and 3'UTR characterized the DENV-3 isolated from both vector and human host as belonging to Genotype III (GIII), despite the differences observed on the 3' UTR. Further studies are needed to address the role of those mutations in the transmission of the different viral populations and vector competence.

Dataset Information

Detection of the Asian II genotype of dengue virus serotype 2 in humans and mosquitoes in Brazil.

Publications

Detection of the Asian II genotype of dengue virus serotype 2 in humans and mosquitoes in Brazil.

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