Ontology highlight
ABSTRACT:
SUBMITTER: Maji B
PROVIDER: S-EPMC7182439 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Maji Basudeb B Gangopadhyay Soumyashree A SA Lee Miseon M Shi Mengchao M Wu Peng P Heler Robert R Mok Beverly B Lim Donghyun D Siriwardena Sachini U SU Paul Bishwajit B Dančík Vlado V Vetere Amedeo A Mesleh Michael F MF Marraffini Luciano A LA Liu David R DR Clemons Paul A PA Wagner Bridget K BK Choudhary Amit A
Cell 20190501 4
The precise control of CRISPR-Cas9 activity is required for a number of genome engineering technologies. Here, we report a generalizable platform that provided the first synthetic small-molecule inhibitors of Streptococcus pyogenes Cas9 (SpCas9) that weigh <500 Da and are cell permeable, reversible, and stable under physiological conditions. We developed a suite of high-throughput assays for SpCas9 functions, including a primary screening assay for SpCas9 binding to the protospacer adjacent moti ...[more]