Silencing of microRNA-135b inhibits invasion, migration, and stemness of CD24⁺CD44⁺ pancreatic cancer stem cells through JADE-1-dependent AKT/mTOR pathway.

ABSTRACT:

Background

Recent studies have emphasized determining the ability of microRNAs (miRNAs) as crucial regulators in the occurrence and development of pancreatic cancer (PC), which continues to be one of the deadliest malignancies with few effective therapies. The study aimed to investigate the functional role of miR-135b and its associated mechanism to unravel the biological characteristics of tumor growth in pancreatic cancer stem cells (PCSCs).

Methods

Microarray analyses were initially performed to identify the PC-related miRNAs and genes. The expression of miR-135b and PCSC markers in PC tissues and cells was determined by RT-qPCR and western blot analysis, respectively. The potential gene (JADE-1) that could bind to miR-135b was confirmed by the dual-luciferase reporter as

SUBMITTER: Zhou J 

PROVIDER: S-EPMC7183669 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature