Ontology highlight
ABSTRACT:
SUBMITTER: Steinmetzger C
PROVIDER: S-EPMC7187157 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20200306 17
RNA aptamers form compact tertiary structures and bind their ligands in specific binding sites. Fluorescence-based strategies reveal information on structure and dynamics of RNA aptamers. Herein, we report the incorporation of the universal emissive nucleobase analog 4-cyanoindole into the fluorogenic RNA aptamer Chili, and its application as a donor for supramolecular FRET to the bound ligands DMHBI<sup>+</sup> or DMHBO<sup>+</sup> . The photophysical properties of the new nucleobase-ligand-FRE ...[more]