Dataset Information

Methane oxidation and methylotroph population dynamics in groundwater mesocosms.

ABSTRACT: Extraction of natural gas from unconventional hydrocarbon reservoirs by hydraulic fracturing raises concerns about methane migration into groundwater. Microbial methane oxidation can be a significant methane sink. Here, we inoculated replicated, sand-packed, continuous mesocosms with groundwater from a field methane release experiment. The mesocosms experienced thirty-five weeks of dynamic methane, oxygen and nitrate concentrations. We determined concentrations and stable isotope signatures of methane, carbon dioxide and nitrate and monitored microbial community composition of suspended and attached biomass. Methane oxidation was strictly dependent on oxygen availability and led to enrichment of 13 C in residual methane. Nitrate did not enhance methane oxidation under oxygen limitation. Methylotrophs persisted for weeks in the absence of methane, making them a powerful marker for active as well as past methane leaks. Thirty-nine distinct populations of methylotrophic bacteria were observed. Methylotrophs mainly occurred attached to sediment particles. Abundances of methanotrophs and other methylotrophs were roughly similar across all samples, pointing at transfer of metabolites from the former to the latter. Two populations of Gracilibacteria (Candidate Phyla Radiation) displayed successive blooms, potentially triggered by a period of methane famine. This study will guide interpretation of future field studies and provides increased understanding of methylotroph ecophysiology.

SUBMITTER: Kuloyo O

PROVIDER: S-EPMC7187433 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Methane oxidation and methylotroph population dynamics in groundwater mesocosms.

Kuloyo Olukayode O Ruff S Emil SE Cahill Aaron A Connors Liam L Zorz Jackie K JK Hrabe de Angelis Isabella I Nightingale Michael M Mayer Bernhard B Strous Marc M

Environmental microbiology 20200207 4

Extraction of natural gas from unconventional hydrocarbon reservoirs by hydraulic fracturing raises concerns about methane migration into groundwater. Microbial methane oxidation can be a significant methane sink. Here, we inoculated replicated, sand-packed, continuous mesocosms with groundwater from a field methane release experiment. The mesocosms experienced thirty-five weeks of dynamic methane, oxygen and nitrate concentrations. We determined concentrations and stable isotope signatures of m ...[more]

PMID: 32017377

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Project description:BackgroundA thorough knowledge of the population dynamics of pests and of the main factors affecting population growth is an important prerequisite for the development of effective control strategies. Failures of various treatments aimed at regulating populations of Dermanyssus gallinae are regularly reported in poultry farms and pullulations occur very quickly after first detection. To finely characterize population dynamics of D. gallinae, and to identify the factors modulating population growth, we conducted two successive multi-generation experiments using closed mesocosms equipped with or without automatic counters and housing a host full- or part-time (three nights per week).ResultsPopulation growth was very rapid and the adult to juvenile ratio very different from the prediction by a mathematical model. A male-biased sex ratio was observed in some mesocosms from 21 days and in most mesocosms from 35 days of population growth originating from an inoculum of adult females. A dramatic slowdown in growth was measured in mesocosms equipped with trackers, where the mites' path to the host was constrained. The slowdown in population growth induced by the intermittent presence of the host compared to its full-time presence was much less marked.ConclusionThese findings suggest avenues of research for new management methods. They question the relevance of a critical threshold based on traditional trap monitoring to manage D. gallinae. Our results highlight a unique characteristic of D. gallinae that makes it a recalcitrant case to threshold-based practices recommended for integrated pest management (IPM) against other arthropod pests. The dramatic effect of a physical constraint for the mite to access the host (unnatural constrained path) confirms an observation made in 1917 and is a reason to design perches that are less conducive to parasite traffic. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Dataset Information

Methane oxidation and methylotroph population dynamics in groundwater mesocosms.

Publications

Methane oxidation and methylotroph population dynamics in groundwater mesocosms.

Similar Datasets

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