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Comparison of the diagnostic methods on the canine adenovirus type 2 infection.


ABSTRACT: Background and aims: Canine adenovirus type 2 (CAV-2) infection is typically diagnosed histopathologically since intranuclear inclusion bodies (IN/IBs) are demonstrable in the infected lung. However, it is sometimes difficult to identify IN/IBs, particularly in autolyzed tissues or samples from both early and late stages of infection, and other methods were presently developed. Methods: Stray dog samples were evaluated by histopathology, polymerase chain reaction (PCR), and immunohistochemistry (IHC) to investigate the status of the CAV-2 infection on the stray dogs in Korea. Histologic tests were performed, and dogs with pneumonic lungs were further evaluated by IHC and PCR. Results: Pathognomonic IN/IBs were identified in 3 of 213 lungs; CAV-2 PCR was positive for 27 of 213 pneumonic lungs. A total of 7 of 27 CAV-2 PCR-positive lungs were IHC-positive. No PCR-negative lung was IHC-positive. Positive results were primarily detected in the IN/IBs of the bronchial epithelial cells, macrophages, and very rarely in the cytoplasm of bronchial epithelial cells. Conclusions: IHC was a more reliable diagnostic method than conventional pathologic methods in the present study, and suggests that IHC should be routinely used in the diagnosis of CAV-2 infection. Further, PCR alone may not be adequate for CAV-2 diagnosis.

SUBMITTER: Yoon SS 

PROVIDER: S-EPMC7188356 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Comparison of the diagnostic methods on the canine adenovirus type 2 infection.

Yoon Soon-Seek SS   Byun Jae-Won JW   Park Young-Il YI   Kim Min-Jeong MJ   Bae You-Chan YC   Song Jae-Young JY  

Basic and applied pathology 20100602 2


<b>Background and aims:</b> Canine adenovirus type 2 (CAV-2) infection is typically diagnosed histopathologically since intranuclear inclusion bodies (IN/IBs) are demonstrable in the infected lung. However, it is sometimes difficult to identify IN/IBs, particularly in autolyzed tissues or samples from both early and late stages of infection, and other methods were presently developed. <b>Methods:</b> Stray dog samples were evaluated by histopathology, polymerase chain reaction (PCR), and immunoh  ...[more]

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