Emodin in Rheum undulatum inhibits oxidative stress in the liver via AMPK with Hippo/Yap signalling pathway.

ABSTRACT: Context: Emodin is a compound in Rheum undulatum Linne (Polygonaceae) that has been reported to exert anti-inflammatory, antibacterial, and antiallergic effects.Objective: Oxidative stress is a causative agent of liver inflammation that may lead to fibrosis and hepato-carcinoma. In this study, we investigated the antioxidant effects of emodin and its mechanism.Materials and methods: We used the hepatocyte stimulated by arachidonic acid (AA)?+?iron cotreatment and the C57B/6 mice orally injected with acetaminophen (APAP, 500?mg/kg, 6?h), as assessed by immunoblot and next generation sequencing (NGS). Emodin was pre-treated in hepatocyte (3???30??M) for 1?h before AA?+?iron, and in mice (10 and 30?m/kg, P.O.) for 3?days before APAP.Results: In vitro, emodin treatment inhibited the cell death induced by AA?+?iron maximally at a dose of 10??M (EC50 > 3??M). In addition, emodin attenuated the decrease of anti-apoptotic proteins, and restored mitochondria membrane potential as mediated by the liver kinase B1 (LKB1)-AMP-activated protein kinase (AMPK) pathway. LKB1 mediated AMPK activation was verified using the LKB1 deficient cell line, HeLa. Emodin (10??M; after 10?min) also induced the phosphorylation of Yes-associated protein 1 (YAP1), the main downstream target of the Hippo signalling pathway that mediated oxidative stress or the ROS-initiated signalling pathway. In vivo, the oral treatment of emodin (10 and 30?m/kg, 3?days) decreased APAP-induced hepatic damage, as indicated by decreases in antioxidant genes as well as tissue damage.Conclusion: Our results show that emodin inhibits oxidative liver injury via the AMPK/YAP mediated pathway.

SUBMITTER: Lee EH 

PROVIDER: S-EPMC7191907 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature