Dataset Information

Development and Application of Mass Spectroscopy Assays for N?-(1-Carboxymethyl)-L-Lysine and Pentosidine in Renal Failure and Diabetes.

ABSTRACT:

Background

Advanced glycation end products (AGEs) are formed via the nonenzymatic glycation of sugars with amino acids. Two AGEs, N?-(1-carboxymethyl)-L-Lysine (CML) and pentosidine, have been observed to be elevated in subjects suffering from a multitude of chronic disease states, and accumulation of these compounds may be related to the pathophysiology of disease progression and aging.

Methods

We describe here the development and validation of a specific and reproducible LC-MS/MS method to quantify CML and pentosidine in human serum with lower limits of quantitation of 75?ng/mL and 5?ng/mL, respectively. The analyte calibration curve exhibited excellent linearity at a range of 0-10 900?ng/mL for CML and 0-800?ng/mL for pentosidine. High-low linearity of 5 serum pairs was assessed, with a mean recovery of 103% (range 94-116%) for CML, and 104% (range 97-116%) for pentosidine.

Results

Serum concentrations of CML and pentosidine were quantified in 30 control and 30 subjects with chronic renal insufficiency. A significant increase in both analytes was observed in renal failure compared to control subjects (2.1-fold and 8.4-fold, respectively; P?ConclusionsThese mass spectroscopy-based assays for serum CML and pentosidine should be useful in accurately evaluating circulating levels of these key AGEs in various disease states.

SUBMITTER: O'Grady KL

PROVIDER: S-EPMC7192546 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Publications

Development and Application of Mass Spectroscopy Assays for Nε-(1-Carboxymethyl)-L-Lysine and Pentosidine in Renal Failure and Diabetes.

O'Grady Katherine L KL Khosla Sundeep S Farr Joshua N JN Bondar Olga P OP Atkinson Elizabeth J EJ Achenbach Sara J SJ Eckhardt Brittany A BA Thicke Brianne S BS Tweed Amanda J AJ Volkman Tammie L TL Drake Matthew T MT Hines Jolaine M JM Singh Ravinder J RJ

The journal of applied laboratory medicine 20200501 3

<h4>Background</h4>Advanced glycation end products (AGEs) are formed via the nonenzymatic glycation of sugars with amino acids. Two AGEs, Nε-(1-carboxymethyl)-L-Lysine (CML) and pentosidine, have been observed to be elevated in subjects suffering from a multitude of chronic disease states, and accumulation of these compounds may be related to the pathophysiology of disease progression and aging.<h4>Methods</h4>We describe here the development and validation of a specific and reproducible LC-MS/M ...[more]

PMID: 32445362

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