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Frame-shifted proteins of a given gene retain the same function.

ABSTRACT: Frameshift mutations are generally considered to be lethal because it could result in radical changes of the protein sequence behind. However, the protein of frameshift mutants of a type I toxin (ibsc) was found to be still toxic to bacteria, retaining the similar function as wild-type protein to arrest the cellular growth by impairing the membrane's integrity. Additionally, we have verified that this observation is not an individual event as the same phenomenon had been found in other toxins subsequently. After analyzing the coding sequence of these genes, we proposed a hypothesis to search this kind of hidden gene, through which a dihydrofolate reductase-encoding gene (dfrB3) was found out. Like the wild-type reductase, both +1 and -1 frame-shifted proteins of dfrB3 gene were also proved to catalyze the reduction of dihydrofolate to tetrahydrofolate by using NADPH.

SUBMITTER: Huang X

PROVIDER: S-EPMC7192591 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Frame-shifted proteins of a given gene retain the same function.

Huang Xin X Chen Rong R Sun Meiling M Peng Yan Y Pu Qinlin Q Yuan Yi Y Chen Gangyi G Dong Juan J Du Feng F Cui Xin X Tang Zhuo Z

Nucleic acids research 20200501 8

Frameshift mutations are generally considered to be lethal because it could result in radical changes of the protein sequence behind. However, the protein of frameshift mutants of a type I toxin (ibsc) was found to be still toxic to bacteria, retaining the similar function as wild-type protein to arrest the cellular growth by impairing the membrane's integrity. Additionally, we have verified that this observation is not an individual event as the same phenomenon had been found in other toxins su ...[more]

PMID: 32187359

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