A drug-inducible sex-separation technique for insects.
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ABSTRACT:
SUBMITTER: Kandul NP
PROVIDER: S-EPMC7193620 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Publications
A drug-inducible sex-separation technique for insects.
Kandul Nikolay P NP Liu Junru J Hsu Alexander D AD Hay Bruce A BA Akbari Omar S OS
Nature communications 20200430 1
Here, we describe a drug-inducible genetic system for insect sex-separation that demonstrates proof-of-principle for positive sex selection in D. melanogaster. The system exploits the toxicity of commonly used broad-spectrum antibiotics geneticin and puromycin to kill the non-rescued sex. Sex-specific rescue is achieved by inserting sex-specific introns into the coding sequences of antibiotic-resistance genes. When raised on geneticin-supplemented food, the sex-sorter line establishes 100% posit ...[more]
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