Ontology highlight
ABSTRACT:
SUBMITTER: Morikawa K
PROVIDER: S-EPMC7195411 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Nature communications 20200501 1
Optogenetic genome engineering tools enable spatiotemporal control of gene expression and provide new insight into biological function. Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0. To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide. To prevent background recombination caused by the high sequence similarity in the dimerizatio ...[more]