Dataset Information

Fine mapping of the major anthracnose resistance QTL AnRGO5 in Capsicum chinense 'PBC932'.

ABSTRACT: BACKGROUND:Colletotrichum species are the causal agents of anthracnose, a major disease affecting the yield and quality of pepper (Capsicum spp.). Colletotrichum scovillei is widespread in China, has strong pathogenicity and drug resistance, and causes anthracnose disease in pepper fruits that severely reduces production. Previously, an anti-anthracnose locus AnRGO5 was mapped to the P5 chromosome on the basis of analyses of fruit at the green mature stage. The aim of this study was to narrow down the interval of this locus and identify the gene responsible for conferring resistance. RESULTS:On the basis of results of re-sequencing of Capsicum chinense 'PBC932' and C. annuum '77013', we developed Kompetitive allele-specific PCR (KASPar) markers and insertion-deletion (InDel) markers linked to AnRGO5 at the green mature fruit stage and used them to construct a genetic linkage map (42 markers, 24.4?cM in length). Using data obtained in phenotypic and genotypic analyses of BC4S1, BC4S2, and BC4S3 populations, AnRGO5 was located between the markers P5in-2266-404 and P5in-2268-978 within a physical distance of 164?kb. This region contained five genes, including CA05g17730. CA05g17730 encodes 'R1C-3-like' putative late blight resistance protein homologs. The transcript level of CA05g17730 differed between 'PBC932' and '77013'. The structure of the CA05g17730 gene also differed between 'PBC932' and '77013'. CONCLUSIONS:We narrowed down the QTL interval to a region containing five genes. These results will be useful for further research on the mechanisms of resistance to anthracnose, and for marker assisted selection for anthracnose-resistant capsicum lines.

SUBMITTER: Zhao Y

PROVIDER: S-EPMC7195712 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Fine mapping of the major anthracnose resistance QTL AnR<sub>GO</sub>5 in Capsicum chinense 'PBC932'.

Zhao Yuanyuan Y Liu Yiwei Y Zhang Zhenghai Z Cao Yacong Y Yu Hailong H Ma Wenwen W Zhang Baoxi B Wang Risheng R Gao Jie J Wang Lihao L

BMC plant biology 20200501 1

<h4>Background</h4>Colletotrichum species are the causal agents of anthracnose, a major disease affecting the yield and quality of pepper (Capsicum spp.). Colletotrichum scovillei is widespread in China, has strong pathogenicity and drug resistance, and causes anthracnose disease in pepper fruits that severely reduces production. Previously, an anti-anthracnose locus AnR<sub>GO</sub>5 was mapped to the P5 chromosome on the basis of analyses of fruit at the green mature stage. The aim of this stu ...[more]

PMID: 32357837

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Project description:BackgroundAnthracnose is a fungal disease caused by Colletotrichum spp. that has a significant impact on worldwide pepper production. Colletotrichum scovillei is the most common pathogenic anthracnose-causing species in the Republic of Korea.ResultsThe resistances of 197 pepper (Capsicum chinense) accessions deposited in Korea's National Agrobiodiversity Center were evaluated for their response against the virulent pathogens Colletotrichum acutatum isolate 'KSCa-1' and C. scovillei isolate 'Hana') in the field and in vitro methods for three consecutive years (2018 to 2020). The severity of the disease was recorded and compared between inoculation methods. Six phenotypically resistant pepper accessions were selected based on three years of disease data. All of the selected resistant pepper accessions outperformed the control resistant pepper in terms of resistance (PI 594,137). A genome-wide association study (GWAS) was carried out to identify single nucleotide polymorphisms (SNPs) associated with anthracnose resistance. An association analysis was performed using 53,518 SNPs and the disease score of the 2020 field and in vitro experiment results. Both field and in vitro experiments revealed 25 and 32 significantly associated SNPs, respectively. These SNPs were found on all chromosomes except Ch06 and Ch07 in the field experiment, whereas in the in vitro experiment they were found on all chromosomes except Ch04 and Ch11.ConclusionIn this study, six resistant C. chinense accessions were selected. Additionally, in this study, significantly associated SNPs were found in a gene that codes for a protein kinase receptor, such as serine/threonine-protein kinase, and other genes that are known to be involved in disease resistance. This may strengthen the role of these genes in the development of anthracnose resistance in Capsicum spp. As a result, the SNPs discovered to be strongly linked in this study can be used to identify a potential marker for selecting pepper material resistant to anthracnose, which will assist in the development of resistant varieties.

Dataset Information

Fine mapping of the major anthracnose resistance QTL AnRGO5 in Capsicum chinense 'PBC932'.

Publications

Fine mapping of the major anthracnose resistance QTL AnR<sub>GO</sub>5 in Capsicum chinense 'PBC932'.

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