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Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.

ABSTRACT: Background:Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity. Methods:In this study, we isolated crude polysaccharides from A. hybridus (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 and AHP-M-2, were obtained. The structural characteristics of AHP-M-1 and AHP-M-2 were investigated, and their antioxidant activities were analyzed in vitro. Results:We found that the monosaccharide composition of AHP-M-1 was different from that of AHP-M-2. The molecular weights of AHP-M-1 and AHP-M-2 were 77.625 kDa and 93.325 kDa, respectively. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. For AHP-M-2, the DPPH radical scavenging rate at a concentration of 2 mg/mL was 78.87%, the hydroxyl radical scavenging rate was 39.34%, the superoxide anion radical scavenging rate was 80.2%, and the reduction ability of Fe3+ was approximately 0.90. The total antioxidant capacity per milligram of AHP-M-2 was 6.42, which was higher than that of Vitamin C (Vc). Conclusion:The in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that A. hybridus L. polysaccharide has immense potential as a natural antioxidants.

SUBMITTER: Tang Z

PROVIDER: S-EPMC7195838 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Purification, characterization and antioxidant activities in vitro of polysaccharides from <i>Amaranthus hybridus</i> L.

Tang Zizhong Z Zhou Caixia C Cai Yi Y Tang Yujia Y Sun Wenjun W Yao Huipeng H Zheng Tianrun T Chen Hui H Xiao Yirong Y Shan Zhi Z Bu Tongliang T Wang Xiaoli X Huang Lin L Gou Lin L

PeerJ 20200429

<h4>Background</h4><i>Amaranthus hybridus</i> L. is an annual, erect or less commonly ascending herb that is a member of the <i>Amaranthaceae</i> family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity.<h4>Methods</h4>In this study, we isolated crude polysaccharides from <i>A. hybridus</i> (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 ...[more]

PMID: 32391207

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Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.

Publications

Purification, characterization and antioxidant activities in vitro of polysaccharides from <i>Amaranthus hybridus</i> L.

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