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LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer.

ABSTRACT: BACKGROUND:Long noncoding RNAs (lncRNA) are important in the growth and metastasis of colon cancer. The objective of this study was to describe the potential role of lncRNA NEAT1 in the progression of colon cancer. METHODS:Quantitative real-time polymerase chain reaction was used for detecting NEAT1, miR-185-5p, and IGF2 in colon cancer cells and tissues. The potential diagnostic value of NEAT1 in colon cancer was analyzed with the receiver operating characteristic curve. Kaplan-Meier method was applied for evaluating the association between NEAT1 expression and the overall survival of osteosarcoma patients, whereas Transwell assay was introduced to examine the potential invasion and migration of colon cancer cells. In addition, the binding of NEAT1/IGF2 to miR-185-5p was confirmed by RNA pull-down and RNA-binding protein immunoprecipitation assays and dual-luciferase reporter gene assay. Finally, rescue experiments were conducted to confirm the role of NEAT1/miR-185-5p/IGF2 axis in colon cancer. RESULTS:Colon cancer patients with low NEAT1 expression presented with longer overall survival than those with high expression. The migration and invasion of colon cancer cells were considerably promoted by overexpressed NEAT1. Both NEAT1 and IGF2 bound to miR-185-5p. CONCLUSION:NEAT1 upregulate IGF2 expression through absorbing miR-185-5p to enhances the migration and invasion of colon cancer cells.

SUBMITTER: Zhuang ST

PROVIDER: S-EPMC7196445 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer.

Zhuang Shu-Tong ST Cai Yan-Juan YJ Liu Hong-Peng HP Qin Ying Y Wen Jian-Feng JF

Molecular genetics & genomic medicine 20200220 4

<h4>Background</h4>Long noncoding RNAs (lncRNA) are important in the growth and metastasis of colon cancer. The objective of this study was to describe the potential role of lncRNA NEAT1 in the progression of colon cancer.<h4>Methods</h4>Quantitative real-time polymerase chain reaction was used for detecting NEAT1, miR-185-5p, and IGF2 in colon cancer cells and tissues. The potential diagnostic value of NEAT1 in colon cancer was analyzed with the receiver operating characteristic curve. Kaplan-M ...[more]

PMID: 32077635

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Project description:Retinoblastoma (RB) is the most common intraocular tumor in childhood. Long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NTAT1) has been reported to be related to RB progression. This study aims to study the molecular mechanism of NEAT1 in regulating cell cycle, proliferation, apoptosis, migration, and invasion in RB. The expression levels of NEAT1 and miR-3619-5p were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The protein expression of LIM and SH3 domain protein 1 (LASP1) was measured by western blot. The proliferation of RB cells was analyzed by cell counting kit-8 (CCK-8) and cell colony formation assays. Cell migration and invasion were evaluated by transwell assay. Cell cycle and apoptosis were assessed by flow cytometry analysis. The association between miR-3619-5p and NEAT1 or LASP1 was predicted by starBase 3.0 database and identified by dual-luciferase reporter assay. The effects of NEAT1 knockdown on the tumor growth in vivo were detected by in vivo tumor formation assay. NEAT1 expression was dramatically up-regulated, and miR-3619-5p expression was obviously downregulated in RB tissues and cells compared with control groups. The protein level of LASP1 was obviously increased in RB tissues or cells relative to paracancerous normal tissues or cells, respectively. Functionally, NEAT1 silencing inhibited RB cell migration, invasion, and proliferation, whereas induced cell apoptosis and cell cycle arrest in RB; this phenomenon was partially abolished by miR-3619-5p inhibitor. Mechanistically, NEAT1 acted as a sponge of miR-3619-5p, and miR-3619-5p was associated with LASP1. In addition, NEAT1 knockdown decreased the volume and weight of RB tumor in vivo. Together, NEAT1 silencing repressed cell migration, invasion, and proliferation, whereas induced cell apoptosis and cycle arrest by sponging miR-3619-5p to inhibit LASP1 expression in RB cells. This study may provide a theoretical basis for RB therapy.

Dataset Information

LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer.

Publications

LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer.

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