Interleukin 22 Signaling Regulates Acinar Cell Plasticity to Promote Pancreatic Tumor Development in Mice.

ABSTRACT:

Background & aims

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy that invades surrounding structures and metastasizes rapidly. Although inflammation is associated with tumor formation and progression, little is known about the mechanisms of this connection. We investigate the effects of interleukin (IL) 22 in the development of pancreatic tumors in mice.

Methods

We performed studies with Pdx1-Cre;LSL-Kras^G12D;Trp53^+/-;Rosa26^EYFP/+ (PKCY) mice, which develop pancreatic tumors, and PKCY mice with disruption of IL22 (PKCY Il22^-/-mice). Pancreata were collected at different stages of tumor development and analyzed by immunohistochemistry, immunoblotting, real-time polymerase chain reaction, and flow cytometry. Some

SUBMITTER: Perusina Lanfranca M 

PROVIDER: S-EPMC7197347 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature