Dataset Information

Development of a new macrophage-specific TRAP mouse (Mac^TRAP) and definition of the renal macrophage translational signature.

ABSTRACT: Tissue macrophages play an important role in organ homeostasis, immunity and the pathogenesis of various inflammation-driven diseases. One major challenge has been to selectively study resident macrophages in highly heterogeneous organs such as kidney. To address this problem, we adopted a Translational Ribosome Affinity Purification (TRAP)- approach and designed a transgene that expresses an eGFP-tagged ribosomal protein (L10a) under the control of the macrophage-specific c-fms promoter to generate c-fms-eGFP-L10a transgenic mice (Mac^TRAP). Rigorous characterization found no gross abnormalities in Mac^TRAP mice and confirmed transgene expression across various organs. Immunohistological analyses of Mac^TRAP kidneys identified eGFP-L10a expressing cells in the tubulointerstitial compartment which stained positive for macrophage marker F4/80. Inflammatory challenge led to robust eGFP-L10a upregulation in kidney, confirming Mac^TRAP responsiveness in vivo. We successfully extracted macrophage-specific polysomal RNA from Mac^TRAP kidneys and conducted RNA sequencing followed by bioinformatical analyses, hereby establishing a comprehensive and unique in vivo gene expression and pathway signature of resident renal macrophages. In summary, we created, validated and applied a new, responsive macrophage-specific TRAP mouse line, defining the translational profile of renal macrophages and dendritic cells. This new tool may be of great value for the study of macrophage biology in different organs and various models of injury and disease.

SUBMITTER: Hofmeister A

PROVIDER: S-EPMC7200716 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Development of a new macrophage-specific TRAP mouse (Mac<sup>TRAP</sup>) and definition of the renal macrophage translational signature.

Hofmeister Andreas A Thomaßen Maximilian C MC Markert Sabrina S Marquardt André A Preußner Mathieu M Rußwurm Martin M Schermuly Ralph T RT Steinhoff Ulrich U Gröne Hermann-Josef HJ Hoyer Joachim J Humphreys Benjamin D BD Grgic Ivica I

Scientific reports 20200505 1

Tissue macrophages play an important role in organ homeostasis, immunity and the pathogenesis of various inflammation-driven diseases. One major challenge has been to selectively study resident macrophages in highly heterogeneous organs such as kidney. To address this problem, we adopted a Translational Ribosome Affinity Purification (TRAP)- approach and designed a transgene that expresses an eGFP-tagged ribosomal protein (L10a) under the control of the macrophage-specific c-fms promoter to gene ...[more]

PMID: 32372032

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Dataset Information

Development of a new macrophage-specific TRAP mouse (Mac^TRAP) and definition of the renal macrophage translational signature.

Publications

Development of a new macrophage-specific TRAP mouse (Mac<sup>TRAP</sup>) and definition of the renal macrophage translational signature.

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Dataset Information

Development of a new macrophage-specific TRAP mouse (MacTRAP) and definition of the renal macrophage translational signature.

Publications

Development of a new macrophage-specific TRAP mouse (Mac<sup>TRAP</sup>) and definition of the renal macrophage translational signature.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Development of a new macrophage-specific TRAP mouse (Mac^TRAP) and definition of the renal macrophage translational signature.