Ontology highlight
ABSTRACT:
SUBMITTER: Nesspor TC
PROVIDER: S-EPMC7200789 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Nesspor Thomas C TC Kinealy Kyle K Mazzanti Nicholas N Diem Michael D MD Boye Kevin K Hoffman Hunter H Springer Christine C Sprenkle Justin J Powers Gordon G Jiang Haiyan H La Porte Sherry L SL Ganesan Rajkumar R Singh Sanjaya S Zwolak Adam A
Scientific reports 20200505 1
Generation of bispecific antibodies (BsAbs) having two unique Fab domains requires heterodimerization of the two heavy chains and pairing of each heavy chain with its cognate light chain. An alternative bispecific scaffold (Bipod) comprising an scFv and a Fab on a heterodimeric Fc eliminates the possibility of light chain mispairing. However, unpredictable levels of chain expression and scFv-induced aggregation can complicate purification and reduce the yield of desired Bipod. Here, we describe ...[more]