Antidiabetic Potential of Prosopis farcta Roots: In Vitro Pancreatic Beta Cell Protection, Enhancement of Glucose Consumption, and Bioassay-Guided Fractionation.

ABSTRACT: By using the streptozotocin- (STZ-) induced cytotoxicity in ?-TC3 cells as an assay model, a bioassay-guided fractionation study was employed to isolate and characterize the potential antidiabetic principles of roots of Prosopis farcta. A combination of open column chromatography on reverse-phase silica gel using a water-ethanol gradient (10?:?90 to 100?:?0) followed by HPLC-based fractionation led to an active compound that appears to be composed of carbohydrate/sugar. When cell viability under STZ was reduced to 49.8?±?4% (mean?±?SD), treatment with the active compound at the concentration of 0.5?mg/mL either as a coadministration or a pretreatment improved the viability to 93?±?1.9% and 91.5?±?7%, respectively. The reduction in the mitochondrial membrane potential by STZ (47.34?±?8.9% of control) was similarly recovered to 84.5?±?4.3 (coadministration) and 88?±?5.5% (pretreatment) by the active fraction. The bioassay-guided fractionation, ?-cell protective effect, and increased glucose consumption (up to 1.49-fold increase) in hepatocytes by the extracts and active fraction are also discussed.

SUBMITTER: Shahbazi B 

PROVIDER: S-EPMC7201843 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature