How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
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ABSTRACT:
SUBMITTER: Corley M
PROVIDER: S-EPMC7202378 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:This study aimed to analyze the transcriptomic changes occuring after knockout of alleles encoding for the RNA-binding proteins Roquin-1 and Roquin-2 (DKO), Regnase-1 (KO) or a combination of all three (TKO) in Th1 cells without or after restimulation with anti-CD3/28 antibodies. Data analysis revealed that in unstimulated as well as restimulated T cells of all three knockout genotypes a number of genes is differentially expressed compared to WT cells, with the strongest deregulation seen in TKO cells. A comparison of gene expression changes of all three genotypes indicated that cooperativity of Roquin and Regnase-1 is the prevalent mode of target regulation.
2020-06-23 | GSE152961 | GEO