Isolation and characterization of a novel Escherichia coli O157:H7-specific phage as a biocontrol agent.

ABSTRACT:

Purpose

Escherichia coli O157:H7 is one of the major foodborne pathogens of global public concern. Bacteriophages (phages) have emerged as a promising alternative to antibiotics for controlling pathogenic bacteria. Here, a lytic E. coli O157:H7-specific phage (KFS-EC) was isolated, identified, and characterized to evaluate its potential as a biocontrol agent for E. coli O157:H7.

Methods

KFS-EC was isolated from slaughterhouse in Korea. Morphological analysis, genomic analysis and several physiological tests were performed to identify and characterize the KFS-EC.

Results

A specificity test indicated KFS-EC was strictly specific to E. coli O157:H7 strains among 60 bacterial strains tested. Morphological and phylogenetic analyses confirmed tha

SUBMITTER: Lee C 

PROVIDER: S-EPMC7203308 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature