Analysis of the Gut Microbiota and Inflammatory Factors in mGluR5-Knockout Mice.

ABSTRACT:

Introduction

Accumulating evidence indicates that the glutamatergic system plays an important role in the development of depression. Notably, the antidepressant effect of metabotropic glutamate receptor 5 (mGluR5) modulation is inconsistent across studies. Here, we attempted to identify the involvement of the gut microbiota and inflammation in mGluR5^-/- mice.

Methods

mGluR5^-/- mice and their wild-type littermates were used in our study. We used the open field (OF) and elevated plus maze (EPM) tests to assess anxiety-like behaviors, and we used the two-day forced swim test (FST) and tail suspension test (TST) to test despair-like behaviors. 16S rDNA was used to analyze the gut microbiota. Enzyme-linked immunosorbent assays (ELISAs) were used to measure t

SUBMITTER: Cai G 

PROVIDER: S-EPMC7203659 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature