Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data.
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ABSTRACT:
SUBMITTER: Clark CM
PROVIDER: S-EPMC7204650 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
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Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data.
Clark Chase M CM Costa Maria S MS Conley Erin E Li Emma E Sanchez Laura M LM Murphy Brian T BT
Journal of visualized experiments : JoVE 20190515 147
In order to visualize the relationship between bacterial phylogeny and specialized metabolite production of bacterial colonies growing on nutrient agar, we developed IDBac-a low-cost and high-throughput matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bioinformatics pipeline. IDBac software is designed for non-experts, is freely available, and capable of analyzing a few to thousands of bacterial colonies. Here, we present procedures for the preparation ...[more]
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