Ontology highlight
ABSTRACT:
SUBMITTER: Ratner HK
PROVIDER: S-EPMC7205310 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Ratner Hannah K HK Escalera-Maurer Andrés A Le Rhun Anaïs A Jaggavarapu Siddharth S Wozniak Jessie E JE Crispell Emily K EK Charpentier Emmanuelle E Weiss David S DS
Molecular cell 20190627 3
In addition to defense against foreign DNA, the CRISPR-Cas9 system of Francisella novicida represses expression of an endogenous immunostimulatory lipoprotein. We investigated the specificity and molecular mechanism of this regulation, demonstrating that Cas9 controls a highly specific regulon of four genes that must be repressed for bacterial virulence. Regulation occurs through a protospacer adjacent motif (PAM)-dependent interaction of Cas9 with its endogenous DNA targets, dependent on a non- ...[more]