Dataset Information

Cell cycle inhibitors improve seed storability after priming treatments.

ABSTRACT: Seed priming is a treatment that controls seed water content to partially activate germination processes such as metabolism but prevents full germination of the seeds. The treatment is well known to enhance seed performance, including germination, but sometimes reduces seed storability or longevity as a side effect. Toward developing a novel priming technique that can maintain seed longevity for a longer time period, chemicals that suppress the seed deterioration under a controlled condition were screened from 80 known biologically active compounds contained in the RIKEN NPDepo authentic library using Arabidopsis thaliana seeds. Seeds primed with mimosine, a cell cycle inhibitor, retained higher survival rate after a controlled deterioration treatment compared to seeds primed without the chemical. In addition, other cell cycle inhibitors such as aphidicolin, hydroxyurea and oryzalin had similar effects on the seed storability after priming. Our results suggest that progression of the cell cycle during priming is an important checkpoint that determines the storability of seeds after the treatment.

SUBMITTER: Sano N

PROVIDER: S-EPMC7205848 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Cell cycle inhibitors improve seed storability after priming treatments.

Sano Naoto N Seo Mitsunori M

Journal of plant research 20190112 2

Seed priming is a treatment that controls seed water content to partially activate germination processes such as metabolism but prevents full germination of the seeds. The treatment is well known to enhance seed performance, including germination, but sometimes reduces seed storability or longevity as a side effect. Toward developing a novel priming technique that can maintain seed longevity for a longer time period, chemicals that suppress the seed deterioration under a controlled condition wer ...[more]

PMID: 30637553

Similar Datasets

Project description:The germination process and seedling development are the determining steps in the plant lifecycle that are the most sensitive to adverse environmental conditions. Therefore, this study was conducted to explore the effects of temperature and osmotic potential on germination responses using threshold models and to establish an optimal priming protocol for improving tolerance responses against osmotic stress in early growth stages. The results demonstrated that osmotic stress of - 0.8 MPa significantly influenced the extent, timing, and speed of seed germination. In addition, priming treatments led to an enhanced performance of early growth stages in response to osmotic stress. Based on thermal-time and hydro-time models, the predicted physiological parameters of the median thermal-time at sub-optimal temperature ( θT50=909.09∘C h), the median ceiling temperature for 50% germination (Tc(50) = 39.29 °C), the common base temperature (Tb = 7.88 °C), the constant thermal-time at supra-optimal temperature ( θT = 805.96 °C h), the threshold water potential (Ψb(50) = - 1.13 MPa), and the hydro-time constant ( θH = 56.09 MPa h) quantitatively describe the tolerance threshold of the germination process under different osmotic and temperature conditions. The results also showed that the efficiency of seed treatments depended on the priming conditions, including temperature, duration, and also concentration of the priming agent. However, the treatments of gibberellic acid (5 days, 10 °C, 100 ppm), salicylic acid (5 days, 10 °C, 50 ppm), calcium chloride (3 days, 10 °C, 10 mM), potassium nitrate (3 days, 10 °C, 100 mM), and hydro-priming (3 days, 10 °C) were optimal protocols of each priming method, resulting in an increased seed vigor under osmotic stress. Hence, the predicted biological parameters could easily be applied to determine the physiological changes of germination under environmental factors over time. Also, results suggest that recommended osmo-, hydro-, and hormonal-priming treatments could be efficient methods for ameliorating the osmotic tolerance in the post-priming stages of this plant, especially in arid lands.Supplementary informationThe online version contains supplementary material available at 10.1007/s12298-022-01229-w.

Project description:BackgroundSeed aging in soybean is a serious challenge for agronomic production and germplasm preservation. However, its genetic basis remains largely unclear in soybean. Unraveling the genetic mechanism involved in seed aging, and enhancing seed storability is an imperative goal for soybean breeding. The aim of this study is to identify quantitative trait loci (QTLs) using high-density genetic linkage maps of soybean for seed storability. In this regard, two recombinant inbred line (RIL) populations derived from Zhengyanghuangdou × Meng 8206 (ZM6) and Linhefenqingdou × Meng 8206 (LM6) crosses were evaluated for three seed-germination related traits viz., germination rate (GR), normal seedling length (SL) and normal seedling fresh weight (FW) under natural and artificial aging conditions to map QTLs for seed storability.ResultsA total of 34 QTLs, including 13 QTLs for GR, 11 QTLs for SL and 10 QTLs for FW, were identified on 11 chromosomes with the phenotypic variation ranged from 7.30 to 23.16% under both aging conditions. All these QTLs were novel, and 21 of these QTLs were clustered in five QTL-rich regions on four different chromosomes viz., Chr3, Chr5, Chr17 &Chr18, among them the highest concentration of seven and six QTLs were found in "QTL hotspot A" (Chr17) and "QTL hotspot B" (Chr5), respectively. Furthermore, QTLs within all the five QTL clusters are linked to at least two studied traits, which is also supported by highly significant correlation between the three germination-related traits. QTLs for seed-germination related traits in "QTL hotspot B" were found in both RIL populations and aging conditions, and also QTLs underlying "QTL hotspot A" are identified in both RIL populations under artificial aging condition. These are the stable genomic regions governing the inheritance of seed storability in soybean, and will be the main focus for soybean breeders.ConclusionThis study uncovers the genetic basis of seed storability in soybean. The newly identified QTLs provides valuable information, and will be main targets for fine mapping, candidate gene identification and marker-assisted breeding. Hence, the present study is the first report for the comprehensive and detailed investigation of genetic architecture of seed storability in soybean.

Dataset Information

Cell cycle inhibitors improve seed storability after priming treatments.

Publications

Cell cycle inhibitors improve seed storability after priming treatments.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets